miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27951 3' -53.2 NC_005886.2 + 14133 0.66 0.78722
Target:  5'- aACGuuuCAGCaaaCAAcUGaACCCGCCUGACg -3'
miRNA:   3'- -UGCuu-GUUGg--GUU-AC-UGGGCGGGUUG- -5'
27951 3' -53.2 NC_005886.2 + 12114 0.67 0.734557
Target:  5'- cGCGGAUGACuCCGAUccggucGACgCGCCgAGCa -3'
miRNA:   3'- -UGCUUGUUG-GGUUA------CUGgGCGGgUUG- -5'
27951 3' -53.2 NC_005886.2 + 33045 0.67 0.717053
Target:  5'- aACGGAuCAACUCGAUgaguucugcgucaucGACCgGCCCAu- -3'
miRNA:   3'- -UGCUU-GUUGGGUUA---------------CUGGgCGGGUug -5'
27951 3' -53.2 NC_005886.2 + 24447 0.68 0.656585
Target:  5'- cGCGAGCGcgccGgCCAGUGACgCCGCgCAc- -3'
miRNA:   3'- -UGCUUGU----UgGGUUACUG-GGCGgGUug -5'
27951 3' -53.2 NC_005886.2 + 36515 0.68 0.645255
Target:  5'- uCGAACGACCCGuc--CCCGCCgcuugcgguaaCAACg -3'
miRNA:   3'- uGCUUGUUGGGUuacuGGGCGG-----------GUUG- -5'
27951 3' -53.2 NC_005886.2 + 24186 0.7 0.511191
Target:  5'- gACGAACuggaucgaGACCCGGccACCaCGCCCGAUg -3'
miRNA:   3'- -UGCUUG--------UUGGGUUacUGG-GCGGGUUG- -5'
27951 3' -53.2 NC_005886.2 + 26127 0.71 0.49938
Target:  5'- uGCGGauacgucugcuacGCAAUCCGGUGACaucaCGCCCGcaGCg -3'
miRNA:   3'- -UGCU-------------UGUUGGGUUACUGg---GCGGGU--UG- -5'
27951 3' -53.2 NC_005886.2 + 42150 0.74 0.328592
Target:  5'- cGCGGACAACCCGAUauaagcGACCCaacgguGCgCGACg -3'
miRNA:   3'- -UGCUUGUUGGGUUA------CUGGG------CGgGUUG- -5'
27951 3' -53.2 NC_005886.2 + 26795 0.75 0.281852
Target:  5'- cGCGAAgcCGGCCgUAAUGugCCGUCCAACc -3'
miRNA:   3'- -UGCUU--GUUGG-GUUACugGGCGGGUUG- -5'
27951 3' -53.2 NC_005886.2 + 43597 1.09 0.001366
Target:  5'- aACGAACAACCCAAUGACCCGCCCAACc -3'
miRNA:   3'- -UGCUUGUUGGGUUACUGGGCGGGUUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.