Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27992 | 5' | -50.3 | NC_005886.2 | + | 35887 | 0.66 | 0.923346 |
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Target: 5'- uCCGACGUUGaugacagugaaCAGCCUGCGauuaugccCGAUGAa -3' miRNA: 3'- -GGCUGCAACg----------GUUGGACGU--------GUUGUUc -5' |
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| 27992 | 5' | -50.3 | NC_005886.2 | + | 4968 | 0.66 | 0.923346 |
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Target: 5'- gCGugGUUGCCuuauuguCUUGCAaAGCAGu -3' miRNA: 3'- gGCugCAACGGuu-----GGACGUgUUGUUc -5' |
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| 27992 | 5' | -50.3 | NC_005886.2 | + | 5631 | 0.66 | 0.929343 |
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Target: 5'- uCgGGCGUUGaCAugcCCUGCAauGCAAGg -3' miRNA: 3'- -GgCUGCAACgGUu--GGACGUguUGUUC- -5' |
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| 27992 | 5' | -50.3 | NC_005886.2 | + | 19522 | 0.66 | 0.929343 |
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Target: 5'- aUCG-CGUacugcuucucUGCCGACCUgaugGCACGugGGGc -3' miRNA: 3'- -GGCuGCA----------ACGGUUGGA----CGUGUugUUC- -5' |
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| 27992 | 5' | -50.3 | NC_005886.2 | + | 2103 | 0.66 | 0.929343 |
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Target: 5'- uCUGACGUUaucGCCGgcACCU-CGCAACGc- -3' miRNA: 3'- -GGCUGCAA---CGGU--UGGAcGUGUUGUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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