miRNA: 3'- -GCUCCGGCGGUGGGUGGGUGGCCGGG- -5'
miRNA: 3'- -GCUCCGGCGG--UGGGUGGGUgGCCGgG- -5'
miRNA: 3'- -GCUCC--GGCGGu-----GGGUGg---GUGGCCGGG- -5'
miRNA: 3'- -GCuCCG-GCG-GUGGGUG-----------GGugGCCGGG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27998 | 5' | -65.7 | NC_005887.1 | + | 40766 | 1.1 | 0.000063 |
|
Target: 5'- uCGAGGCCGCCACCCACCCACCGGCCCa -3' miRNA: 3'- -GCUCCGGCGGUGGGUGGGUGGCCGGG- -5' |
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| 27998 | 5' | -65.7 | NC_005887.1 | + | 41464 | 0.74 | 0.055631 |
|
Target: 5'- cCGAGGCCGCCugACUCGUCCGgCGGCgCg -3' miRNA: 3'- -GCUCCGGCGG--UGGGUGGGUgGCCGgG- -5' |
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| 27998 | 5' | -65.7 | NC_005887.1 | + | 41472 | 0.66 | 0.209693 |
|
Target: 5'- gCGAGGauCUGCUcaucauCCCGCauggCACCGGCUCg -3' miRNA: 3'- -GCUCC--GGCGGu-----GGGUGg---GUGGCCGGG- -5' |
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| 27998 | 5' | -65.7 | NC_005887.1 | + | 41973 | 0.68 | 0.142717 |
|
Target: 5'- gCGcGGCgCGCuCGCCUAUcagcagcacggCCugCGGCCCa -3' miRNA: 3'- -GCuCCG-GCG-GUGGGUG-----------GGugGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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