Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28002 | 5' | -62.7 | NC_005887.1 | + | 15317 | 0.69 | 0.167923 |
Target: 5'- aGCGuGCGC-GGUCGCgcgcUCGCCGaGGUUg -3' miRNA: 3'- aCGCuCGCGaCCAGCG----GGCGGC-CUAG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 12332 | 0.68 | 0.218134 |
Target: 5'- aGCGAacuGCGUacGUCGCUCGCCGGc-- -3' miRNA: 3'- aCGCU---CGCGacCAGCGGGCGGCCuag -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 10050 | 0.66 | 0.273793 |
Target: 5'- cUGCGGcGCGCcGGcuggcccgacgaUCGCCCcguguggaCCGGAUCa -3' miRNA: 3'- -ACGCU-CGCGaCC------------AGCGGGc-------GGCCUAG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 8845 | 0.71 | 0.118233 |
Target: 5'- cGCGGGCaGCUugugaacgccGGcgCGCCCGUCGaGAUCg -3' miRNA: 3'- aCGCUCG-CGA----------CCa-GCGGGCGGC-CUAG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 7268 | 0.66 | 0.286207 |
Target: 5'- aGCGAcCGCgacaucggccccGGcCGCCCGCUguGGGUCg -3' miRNA: 3'- aCGCUcGCGa-----------CCaGCGGGCGG--CCUAG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 7198 | 0.66 | 0.287613 |
Target: 5'- cGUGcAGCGUgacGGcCGCCCguccguggcGCCGGAUUc -3' miRNA: 3'- aCGC-UCGCGa--CCaGCGGG---------CGGCCUAG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 6300 | 0.74 | 0.080248 |
Target: 5'- cGCGAGCGCgucgaGGccuugCGCCuCGCCGGcgCc -3' miRNA: 3'- aCGCUCGCGa----CCa----GCGG-GCGGCCuaG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 5790 | 0.66 | 0.287613 |
Target: 5'- cUGCGAagcagGCGCacgaaCGCCUGCCGGAauUCg -3' miRNA: 3'- -ACGCU-----CGCGacca-GCGGGCGGCCU--AG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 4888 | 0.66 | 0.260511 |
Target: 5'- cGCGA-CGaUGGUCGCCgaggGCCGGcgCg -3' miRNA: 3'- aCGCUcGCgACCAGCGGg---CGGCCuaG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 4636 | 0.66 | 0.280636 |
Target: 5'- cGCGAauacGCGCUGaaccugaUCGCCC-CCGGcGUCg -3' miRNA: 3'- aCGCU----CGCGACc------AGCGGGcGGCC-UAG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 4625 | 0.69 | 0.167923 |
Target: 5'- cGCG-GCGCcGGUgG-CCGCCGGcgCg -3' miRNA: 3'- aCGCuCGCGaCCAgCgGGCGGCCuaG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 2954 | 0.66 | 0.280636 |
Target: 5'- cGCGGuCGCgaccacGGUUGCgCCGCCGGccUCg -3' miRNA: 3'- aCGCUcGCGa-----CCAGCG-GGCGGCCu-AG- -5' |
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28002 | 5' | -62.7 | NC_005887.1 | + | 1433 | 0.68 | 0.20185 |
Target: 5'- cGCGAGCuGCUGGaagGCgUuCCGGAUCa -3' miRNA: 3'- aCGCUCG-CGACCag-CGgGcGGCCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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