Results 1 - 20 of 56 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28004 | 5' | -57.7 | NC_005887.1 | + | 38878 | 1.1 | 0.000339 |
Target: 5'- uCGAUCAUCAGCGCGCCAGGCCCGAACa -3' miRNA: 3'- -GCUAGUAGUCGCGCGGUCCGGGCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 32123 | 0.8 | 0.058612 |
Target: 5'- aGGUCAUCAGaCGCGCCGuccGGCgCGGGCa -3' miRNA: 3'- gCUAGUAGUC-GCGCGGU---CCGgGCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 33741 | 0.77 | 0.093175 |
Target: 5'- gCGAggAUCAGCGCGCC-GGCCuucaCGAGCa -3' miRNA: 3'- -GCUagUAGUCGCGCGGuCCGG----GCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 26072 | 0.74 | 0.16361 |
Target: 5'- --cUgGUCAGCGCGUCAgcGGCCCGcGCg -3' miRNA: 3'- gcuAgUAGUCGCGCGGU--CCGGGCuUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 14348 | 0.74 | 0.16361 |
Target: 5'- gCGAUCGUgaagCAGCGUGCCGGGCaCCu--- -3' miRNA: 3'- -GCUAGUA----GUCGCGCGGUCCG-GGcuug -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 21808 | 0.73 | 0.177625 |
Target: 5'- aCGAcgUCcgCGGCGCuGCCGGGCgcgCCGAAUg -3' miRNA: 3'- -GCU--AGuaGUCGCG-CGGUCCG---GGCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 28527 | 0.73 | 0.192177 |
Target: 5'- gCGGUCAggcacgcUCAcGCGCGCCGGGCUgcUGAAUu -3' miRNA: 3'- -GCUAGU-------AGU-CGCGCGGUCCGG--GCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 10038 | 0.72 | 0.214526 |
Target: 5'- uGGUCcuacgcacugCGGCGCGCCGgcuGGCCCGAc- -3' miRNA: 3'- gCUAGua--------GUCGCGCGGU---CCGGGCUug -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 10257 | 0.72 | 0.226217 |
Target: 5'- uGAUCGUCcGC-CGCCAGGUCaaCGGGCa -3' miRNA: 3'- gCUAGUAGuCGcGCGGUCCGG--GCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 12419 | 0.7 | 0.278451 |
Target: 5'- uCGAUCccgagAUC-GUGCGCgAGGCCCGcGGCa -3' miRNA: 3'- -GCUAG-----UAGuCGCGCGgUCCGGGC-UUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 15089 | 0.7 | 0.292928 |
Target: 5'- cCGAUCAUUGGCGaCG-CGGGCCgGcGCg -3' miRNA: 3'- -GCUAGUAGUCGC-GCgGUCCGGgCuUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 38638 | 0.7 | 0.307986 |
Target: 5'- gGAUCGcggUCGGCGCGC--GGCUCGAcgGCg -3' miRNA: 3'- gCUAGU---AGUCGCGCGguCCGGGCU--UG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 23979 | 0.7 | 0.311067 |
Target: 5'- gCGAgCGcCGGCGCGCCGucuacgcggaagucGCCCGAACg -3' miRNA: 3'- -GCUaGUaGUCGCGCGGUc-------------CGGGCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 40755 | 0.69 | 0.323627 |
Target: 5'- gCGAUCgccGUUGGCGCGCCGcGCgccgCCGAGCc -3' miRNA: 3'- -GCUAG---UAGUCGCGCGGUcCG----GGCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 13124 | 0.69 | 0.323627 |
Target: 5'- aGGUCGacaccUCgAGCGUGCCGGGCCuCGucuCg -3' miRNA: 3'- gCUAGU-----AG-UCGCGCGGUCCGG-GCuu-G- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 2424 | 0.69 | 0.323627 |
Target: 5'- -cGUCGUCGaCGCGCCcGGCUCGAucuGCg -3' miRNA: 3'- gcUAGUAGUcGCGCGGuCCGGGCU---UG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 27627 | 0.69 | 0.323627 |
Target: 5'- uCGAUCGUCGccugcgcgaucGcCGCGCCcGGCCCGc-- -3' miRNA: 3'- -GCUAGUAGU-----------C-GCGCGGuCCGGGCuug -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 18876 | 0.69 | 0.331668 |
Target: 5'- aGAUCAagGGCGuCGCC-GGCugCCGGGCu -3' miRNA: 3'- gCUAGUagUCGC-GCGGuCCG--GGCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 20532 | 0.69 | 0.331668 |
Target: 5'- --uUCAuUCGGCGCGCCcggcAGcGCCgCGGACg -3' miRNA: 3'- gcuAGU-AGUCGCGCGG----UC-CGG-GCUUG- -5' |
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28004 | 5' | -57.7 | NC_005887.1 | + | 30438 | 0.69 | 0.339854 |
Target: 5'- cCGA-CGUCGGgGCggggcauguuGCCGGGCUgGAACa -3' miRNA: 3'- -GCUaGUAGUCgCG----------CGGUCCGGgCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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