Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28007 | 5' | -53.5 | NC_005887.1 | + | 37702 | 1.13 | 0.000594 |
Target: 5'- cUGCGCGUCGCUGAGAUCCUUGAUCGCg -3' miRNA: 3'- -ACGCGCAGCGACUCUAGGAACUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 30906 | 0.79 | 0.146061 |
Target: 5'- cUGCGCGUUGC---GAUCCaUGAUCGCg -3' miRNA: 3'- -ACGCGCAGCGacuCUAGGaACUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 6013 | 0.73 | 0.360089 |
Target: 5'- gGUGCGUCGCUGAGugaagggguuuugaAUCCgggUGGU-GCu -3' miRNA: 3'- aCGCGCAGCGACUC--------------UAGGa--ACUAgCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 29896 | 0.71 | 0.468769 |
Target: 5'- cGCGCGUCgaaccguacagGCUcGGGUCCaucgcGAUCGCg -3' miRNA: 3'- aCGCGCAG-----------CGAcUCUAGGaa---CUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 27652 | 0.69 | 0.543872 |
Target: 5'- uUGUGCG-CGCUGAGcugCCgcgcgcgcggcUUGAUCGUc -3' miRNA: 3'- -ACGCGCaGCGACUCua-GG-----------AACUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 38151 | 0.69 | 0.554949 |
Target: 5'- cUGCGCG-CGCUGcAGca-CUUcGGUCGCg -3' miRNA: 3'- -ACGCGCaGCGAC-UCuagGAA-CUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 30624 | 0.68 | 0.599828 |
Target: 5'- aGCGCGUC-CgGcAGAUCCg-GAUCGUu -3' miRNA: 3'- aCGCGCAGcGaC-UCUAGGaaCUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 4633 | 0.68 | 0.599827 |
Target: 5'- cUGCGCGaauacgCGCUGAa--CCU-GAUCGCc -3' miRNA: 3'- -ACGCGCa-----GCGACUcuaGGAaCUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 27028 | 0.67 | 0.690298 |
Target: 5'- cGCGUGUCGC-GAGGUaaUUGuucaCGCg -3' miRNA: 3'- aCGCGCAGCGaCUCUAggAACua--GCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 9414 | 0.67 | 0.656497 |
Target: 5'- cGCGCgGUCGUUGAGAcguauggCCUgaaUGGUCu- -3' miRNA: 3'- aCGCG-CAGCGACUCUa------GGA---ACUAGcg -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 12577 | 0.67 | 0.656497 |
Target: 5'- cUGCGCGaCGCgGcGAUCCUcgcGAUCaGCu -3' miRNA: 3'- -ACGCGCaGCGaCuCUAGGAa--CUAG-CG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 6297 | 0.67 | 0.690298 |
Target: 5'- gUGCGCGagCGCgucGAGG-CCUUGcgccUCGCc -3' miRNA: 3'- -ACGCGCa-GCGa--CUCUaGGAACu---AGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 26463 | 0.67 | 0.698119 |
Target: 5'- cUGCGCGUCgagcgcaccgagcuGCUGGuGcgCCUgcUGcgCGCg -3' miRNA: 3'- -ACGCGCAG--------------CGACU-CuaGGA--ACuaGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 12627 | 0.67 | 0.701462 |
Target: 5'- cGCGCGgCGCgu-GAUCCUgcGA-CGCg -3' miRNA: 3'- aCGCGCaGCGacuCUAGGAa-CUaGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 39783 | 0.66 | 0.745276 |
Target: 5'- gGCGC-UCGCgccGAUCgUUGcgCGCg -3' miRNA: 3'- aCGCGcAGCGacuCUAGgAACuaGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 36934 | 0.66 | 0.742047 |
Target: 5'- cGCGCcgGUCGCUGcGGcgCCgcccgccgguucaccGGUCGCg -3' miRNA: 3'- aCGCG--CAGCGAC-UCuaGGaa-------------CUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 5502 | 0.66 | 0.745276 |
Target: 5'- cGCGCGUCGC---GAUCCag---CGCa -3' miRNA: 3'- aCGCGCAGCGacuCUAGGaacuaGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 15565 | 0.66 | 0.755957 |
Target: 5'- cGCGCGaccgCGCUc-GAUUCgcUGAUCGCc -3' miRNA: 3'- aCGCGCa---GCGAcuCUAGGa-ACUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 37490 | 0.66 | 0.755957 |
Target: 5'- aGgGCGgCGgUcAGuUCCUUGAUCGCc -3' miRNA: 3'- aCgCGCaGCgAcUCuAGGAACUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 22989 | 0.66 | 0.723563 |
Target: 5'- gGCGCGUugcCGCUGAucGAcgCC--GGUCGCu -3' miRNA: 3'- aCGCGCA---GCGACU--CUa-GGaaCUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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