Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28007 | 5' | -53.5 | NC_005887.1 | + | 22989 | 0.66 | 0.723563 |
Target: 5'- gGCGCGUugcCGCUGAucGAcgCC--GGUCGCu -3' miRNA: 3'- aCGCGCA---GCGACU--CUa-GGaaCUAGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 24950 | 0.66 | 0.712554 |
Target: 5'- cGUGCG-CGC-GGGccgCCUUGcgCGCg -3' miRNA: 3'- aCGCGCaGCGaCUCua-GGAACuaGCG- -5' |
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28007 | 5' | -53.5 | NC_005887.1 | + | 36934 | 0.66 | 0.742047 |
Target: 5'- cGCGCcgGUCGCUGcGGcgCCgcccgccgguucaccGGUCGCg -3' miRNA: 3'- aCGCG--CAGCGAC-UCuaGGaa-------------CUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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