miRNA: 3'- aCGCGcAGCGacuCUAGgAACuaGCG- -5'
miRNA: 3'- aCGCGCa---GCGAcuCUAGGa-ACUAGCG- -5'
miRNA: 3'- aCgCGCaGCgAcUCuAGGAACUAGCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28007 | 5' | -53.5 | NC_005887.1 | + | 39783 | 0.66 | 0.745276 |
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Target: 5'- gGCGC-UCGCgccGAUCgUUGcgCGCg -3' miRNA: 3'- aCGCGcAGCGacuCUAGgAACuaGCG- -5' |
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| 28007 | 5' | -53.5 | NC_005887.1 | + | 15565 | 0.66 | 0.755957 |
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Target: 5'- cGCGCGaccgCGCUc-GAUUCgcUGAUCGCc -3' miRNA: 3'- aCGCGCa---GCGAcuCUAGGa-ACUAGCG- -5' |
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| 28007 | 5' | -53.5 | NC_005887.1 | + | 37490 | 0.66 | 0.755957 |
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Target: 5'- aGgGCGgCGgUcAGuUCCUUGAUCGCc -3' miRNA: 3'- aCgCGCaGCgAcUCuAGGAACUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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