miRNA: 3'- aCGCGCAGCGACUC--------------UAGGa--ACUAgCG- -5'
miRNA: 3'- -ACGCGCAGCGacuCUAGGaACUAGCG- -5'
miRNA: 3'- -ACGCGCAGCGACUCUAGGAACUAGCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28007 | 5' | -53.5 | NC_005887.1 | + | 6013 | 0.73 | 0.360089 |
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Target: 5'- gGUGCGUCGCUGAGugaagggguuuugaAUCCgggUGGU-GCu -3' miRNA: 3'- aCGCGCAGCGACUC--------------UAGGa--ACUAgCG- -5' |
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| 28007 | 5' | -53.5 | NC_005887.1 | + | 30906 | 0.79 | 0.146061 |
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Target: 5'- cUGCGCGUUGC---GAUCCaUGAUCGCg -3' miRNA: 3'- -ACGCGCAGCGacuCUAGGaACUAGCG- -5' |
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| 28007 | 5' | -53.5 | NC_005887.1 | + | 37702 | 1.13 | 0.000594 |
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Target: 5'- cUGCGCGUCGCUGAGAUCCUUGAUCGCg -3' miRNA: 3'- -ACGCGCAGCGACUCUAGGAACUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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