Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28011 | 5' | -56.6 | NC_005887.1 | + | 24944 | 0.75 | 0.151797 |
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Target: 5'- -gCGACGCGUGCGCgCGGGCcgccuuGCGcGCGg -3' miRNA: 3'- uaGCUGUGCACGUG-GCUCGu-----UGC-CGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 12630 | 0.76 | 0.135709 |
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Target: 5'- -gCGGCGCGUGaucCugCGAcGCGACGGCGa -3' miRNA: 3'- uaGCUGUGCAC---GugGCU-CGUUGCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 36514 | 0.78 | 0.105097 |
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Target: 5'- gGUCGAC-CGcaGCAUCGGGCGGCGGCa -3' miRNA: 3'- -UAGCUGuGCa-CGUGGCUCGUUGCCGc -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 21254 | 0.78 | 0.093684 |
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Target: 5'- cAUCGACccuCGgggGCACCGcGGCGGCGGCGu -3' miRNA: 3'- -UAGCUGu--GCa--CGUGGC-UCGUUGCCGC- -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 35560 | 0.79 | 0.085657 |
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Target: 5'- cUCGACAUGUGCggcgagcGCCGGGCAcACGGCc -3' miRNA: 3'- uAGCUGUGCACG-------UGGCUCGU-UGCCGc -5' |
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| 28011 | 5' | -56.6 | NC_005887.1 | + | 33734 | 1.07 | 0.000684 |
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Target: 5'- gAUCGACACGUGCACCGAGCAACGGCGa -3' miRNA: 3'- -UAGCUGUGCACGUGGCUCGUUGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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