Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28013 | 3' | -55.6 | NC_005887.1 | + | 36827 | 0.67 | 0.581018 |
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Target: 5'- aUCGuGCGuGCCUGcugcgGCCGGUGCuGCg -3' miRNA: 3'- -AGCcUGC-CGGACuua--UGGCUACG-CGa -5' |
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| 28013 | 3' | -55.6 | NC_005887.1 | + | 11827 | 0.67 | 0.585457 |
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Target: 5'- cUCGGccgacggccgccuacGCGGgCUGAugcaguuUugCGGUGCGCa -3' miRNA: 3'- -AGCC---------------UGCCgGACUu------AugGCUACGCGa -5' |
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| 28013 | 3' | -55.6 | NC_005887.1 | + | 11027 | 0.66 | 0.614443 |
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Target: 5'- cCGGuCGGCacgugggacgUGAccACCGGUGCGCc -3' miRNA: 3'- aGCCuGCCGg---------ACUuaUGGCUACGCGa -5' |
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| 28013 | 3' | -55.6 | NC_005887.1 | + | 11087 | 0.66 | 0.614443 |
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Target: 5'- aUCGacGCGGCCgacGAGUACUGGUG-GCa -3' miRNA: 3'- -AGCc-UGCCGGa--CUUAUGGCUACgCGa -5' |
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| 28013 | 3' | -55.6 | NC_005887.1 | + | 29161 | 0.66 | 0.636821 |
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Target: 5'- gUCGGccgGCGGCCUGcucgACCGcucccgcugucUGCGCg -3' miRNA: 3'- -AGCC---UGCCGGACuua-UGGCu----------ACGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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