Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28018 | 3' | -58.7 | NC_005887.1 | + | 5119 | 0.72 | 0.19765 |
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Target: 5'- aGGUGCUcgccaaGCUCGGCaugaccgcgCUGCCcgACGUCGgCg -3' miRNA: 3'- -CCACGA------CGAGCCG---------GACGG--UGCAGUgG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 41608 | 0.72 | 0.192458 |
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Target: 5'- aGGUGCgGCggccggcgCGGCCcgugaggaucUGCUGCGUCugCg -3' miRNA: 3'- -CCACGaCGa-------GCCGG----------ACGGUGCAGugG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 16387 | 0.72 | 0.187386 |
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Target: 5'- aGUGUcgaagaUGCUCGGCguCUGCCGgGcCACCg -3' miRNA: 3'- cCACG------ACGAGCCG--GACGGUgCaGUGG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 38158 | 0.73 | 0.172871 |
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Target: 5'- --cGCUGCagcacuUCGGUCgcgGUCAUGUCGCCg -3' miRNA: 3'- ccaCGACG------AGCCGGa--CGGUGCAGUGG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 36872 | 0.75 | 0.111264 |
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Target: 5'- cGGUGCUGCgccaUCGGCCUGCguCGUgGu- -3' miRNA: 3'- -CCACGACG----AGCCGGACGguGCAgUgg -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 30522 | 0.76 | 0.101986 |
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Target: 5'- cGUGCUGCUCGagaacaggccgccGCCUGCCuuaGCGUacgaACCg -3' miRNA: 3'- cCACGACGAGC-------------CGGACGG---UGCAg---UGG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 18406 | 0.77 | 0.091349 |
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Target: 5'- cGGUGCgUGCgcagaCGGCCgcgagGCCgACGUCAUCg -3' miRNA: 3'- -CCACG-ACGa----GCCGGa----CGG-UGCAGUGG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 28715 | 0.87 | 0.016444 |
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Target: 5'- cGGUGCUGCUCGGCUgagugcggccGCCGCG-CGCCg -3' miRNA: 3'- -CCACGACGAGCCGGa---------CGGUGCaGUGG- -5' |
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| 28018 | 3' | -58.7 | NC_005887.1 | + | 29984 | 1.14 | 0.000138 |
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Target: 5'- cGGUGCUGCUCGGCCUGCCACGUCACCg -3' miRNA: 3'- -CCACGACGAGCCGGACGGUGCAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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