Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28020 | 5' | -51.2 | NC_005887.1 | + | 28516 | 1.11 | 0.001682 |
Target: 5'- gGGAUUUCGUGCCCGUUCGCCAACACGc -3' miRNA: 3'- -CCUAAAGCACGGGCAAGCGGUUGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 34333 | 0.76 | 0.342372 |
Target: 5'- aGGGUUUUGUG-CCGUggCGCCGGcCACGg -3' miRNA: 3'- -CCUAAAGCACgGGCAa-GCGGUU-GUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 9550 | 0.73 | 0.496456 |
Target: 5'- ---gUUCGUGCCCGUgaagcggcgcgCGCCGauccugcGCACGa -3' miRNA: 3'- ccuaAAGCACGGGCAa----------GCGGU-------UGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 22772 | 0.72 | 0.529022 |
Target: 5'- -----aCGUGCUCGUUggcucgaCGCCGGCGCGg -3' miRNA: 3'- ccuaaaGCACGGGCAA-------GCGGUUGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 39468 | 0.72 | 0.541163 |
Target: 5'- -----aCGUGUCCGUcgUCGCCuGCGCGu -3' miRNA: 3'- ccuaaaGCACGGGCA--AGCGGuUGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 21046 | 0.72 | 0.572456 |
Target: 5'- cGGUgggCGUGCCCGUgaaugugggcgaCGCCAGCAgCGc -3' miRNA: 3'- cCUAaa-GCACGGGCAa-----------GCGGUUGU-GC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 24770 | 0.72 | 0.574709 |
Target: 5'- gGGAUUgacgggCGUGUUCGUcgCGCCGGuCACGu -3' miRNA: 3'- -CCUAAa-----GCACGGGCAa-GCGGUU-GUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 26109 | 0.7 | 0.665661 |
Target: 5'- ------gGUGCCCGUgcgcUCGCCGGC-CGg -3' miRNA: 3'- ccuaaagCACGGGCA----AGCGGUUGuGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 7301 | 0.7 | 0.665661 |
Target: 5'- uGGGUcgUCGUGUcgCCGUccgUCGCCGccgacaACACGa -3' miRNA: 3'- -CCUAa-AGCACG--GGCA---AGCGGU------UGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 13994 | 0.7 | 0.672467 |
Target: 5'- cGcgUUCGUGCUCGaagccaaugccgCGCCGGCGCa -3' miRNA: 3'- cCuaAAGCACGGGCaa----------GCGGUUGUGc -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 33051 | 0.7 | 0.687159 |
Target: 5'- cGGcGUUUCgGUGCCCGUaaccugcggcgUCGCCGugccgaccgccguGCACa -3' miRNA: 3'- -CC-UAAAG-CACGGGCA-----------AGCGGU-------------UGUGc -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 24675 | 0.69 | 0.743617 |
Target: 5'- ----aUCGUGCCgaCG-UCGCCAAgCGCGg -3' miRNA: 3'- ccuaaAGCACGG--GCaAGCGGUU-GUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 35965 | 0.69 | 0.743617 |
Target: 5'- ----cUCGUGCCC-UUCGCCc-CGCGu -3' miRNA: 3'- ccuaaAGCACGGGcAAGCGGuuGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 6781 | 0.68 | 0.754371 |
Target: 5'- aGGccg-UGUGCCCGgcgcUCGCCGcACAUGu -3' miRNA: 3'- -CCuaaaGCACGGGCa---AGCGGU-UGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 7965 | 0.68 | 0.764991 |
Target: 5'- cGGGccUCGcGCCCGgcCGCCucugauACGCGg -3' miRNA: 3'- -CCUaaAGCaCGGGCaaGCGGu-----UGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 12326 | 0.68 | 0.775465 |
Target: 5'- aGGGUggcgUCG-GCgCGUUCGUCAcguuCACGa -3' miRNA: 3'- -CCUAa---AGCaCGgGCAAGCGGUu---GUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 1594 | 0.67 | 0.805882 |
Target: 5'- uGGGUggCG-GCCuCGaacCGCCAACGCa -3' miRNA: 3'- -CCUAaaGCaCGG-GCaa-GCGGUUGUGc -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 37942 | 0.67 | 0.805882 |
Target: 5'- aGGAUcgcCGcGCCU--UCGCCGGCGCGc -3' miRNA: 3'- -CCUAaa-GCaCGGGcaAGCGGUUGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 235 | 0.67 | 0.805882 |
Target: 5'- aGGAUcgccacaaGUcCUCGUUCGUCGACGCGg -3' miRNA: 3'- -CCUAaag-----CAcGGGCAAGCGGUUGUGC- -5' |
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28020 | 5' | -51.2 | NC_005887.1 | + | 28451 | 0.67 | 0.834542 |
Target: 5'- -----aCGUGCCaCGaaUGCCGACGCGc -3' miRNA: 3'- ccuaaaGCACGG-GCaaGCGGUUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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