Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28020 | 5' | -51.2 | NC_005887.1 | + | 24113 | 0.67 | 0.843653 |
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Target: 5'- ------gGUGCCCGgcgUgGCCGAgGCGa -3' miRNA: 3'- ccuaaagCACGGGCa--AgCGGUUgUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 7016 | 0.66 | 0.852526 |
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Target: 5'- ----cUCGUGgCCGgcCGCCGcggcGCGCGg -3' miRNA: 3'- ccuaaAGCACgGGCaaGCGGU----UGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 24211 | 0.66 | 0.86115 |
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Target: 5'- cGAcgUCGgcgGCCCGcgcUUCGCCGguugcguacaGCGCGu -3' miRNA: 3'- cCUaaAGCa--CGGGC---AAGCGGU----------UGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 28568 | 0.66 | 0.86115 |
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Target: 5'- aGGcg-UCGccGCCCGcugCGCgGACGCGg -3' miRNA: 3'- -CCuaaAGCa-CGGGCaa-GCGgUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 2172 | 0.66 | 0.86115 |
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Target: 5'- uGGAcUUCG-GCCUGa-CGCCGGcCGCGg -3' miRNA: 3'- -CCUaAAGCaCGGGCaaGCGGUU-GUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 38867 | 0.66 | 0.869518 |
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Target: 5'- cGAcgUCGUGCUCGaUCaUCAGCGCGc -3' miRNA: 3'- cCUaaAGCACGGGCaAGcGGUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 31206 | 0.66 | 0.885452 |
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Target: 5'- aGAUUUCGggcGCCgCGUgcuucaGCaCGACGCGa -3' miRNA: 3'- cCUAAAGCa--CGG-GCAag----CG-GUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 18839 | 0.66 | 0.885453 |
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Target: 5'- --------cGCgCGUUCGUCAGCGCGg -3' miRNA: 3'- ccuaaagcaCGgGCAAGCGGUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 26150 | 0.66 | 0.893006 |
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Target: 5'- -----gCGUGUCCGgUCGC-AGCGCGg -3' miRNA: 3'- ccuaaaGCACGGGCaAGCGgUUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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