miRNA: 3'- gcACGUuAGCUGCAuUGGCUacAGCU- -5'
miRNA: 3'- -GCACGUuaGCUGCa-UUGGCua-----CAGCU- -5'
miRNA: 3'- -GCACg---------UUAGCUGCaUUGGCUaCAGCU- -5'
miRNA: 3'- -GCA-CGU---UAGC----UGCAU--UGGCUACAGcu -5'
miRNA: 3'- -GCACGUUAGCUGCAUUGGCUACAGCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28022 | 3' | -48.9 | NC_005887.1 | + | 1415 | 0.66 | 0.948185 |
|
Target: 5'- -aUGCGcUCGGCGUuGCCGAacUCGGu -3' miRNA: 3'- gcACGUuAGCUGCAuUGGCUacAGCU- -5' |
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| 28022 | 3' | -48.9 | NC_005887.1 | + | 13961 | 0.66 | 0.948185 |
|
Target: 5'- aCGUGCGcgUGACGacAGCCGcgaagccGUCGAc -3' miRNA: 3'- -GCACGUuaGCUGCa-UUGGCua-----CAGCU- -5' |
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| 28022 | 3' | -48.9 | NC_005887.1 | + | 15222 | 0.66 | 0.952861 |
|
Target: 5'- gCGUGaucgccgacgAGUCGACGaAGCUGAaGUCGAc -3' miRNA: 3'- -GCACg---------UUAGCUGCaUUGGCUaCAGCU- -5' |
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| 28022 | 3' | -48.9 | NC_005887.1 | + | 35542 | 0.66 | 0.957244 |
|
Target: 5'- aCGUaGCGccaGUCGcgcaGCGUGagGCCGAUGUCc- -3' miRNA: 3'- -GCA-CGU---UAGC----UGCAU--UGGCUACAGcu -5' |
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| 28022 | 3' | -48.9 | NC_005887.1 | + | 28177 | 1.1 | 0.003527 |
|
Target: 5'- gCGUGCAAUCGACGUAACCGAUGUCGAc -3' miRNA: 3'- -GCACGUUAGCUGCAUUGGCUACAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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