Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28028 | 3' | -54.7 | NC_005887.1 | + | 38252 | 0.68 | 0.538842 |
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Target: 5'- cCUGCGCGaacucgaugCCGAUCUGcgccugcUUGCCcguGAGCAg -3' miRNA: 3'- -GACGCGUa--------GGCUAGGC-------AACGG---UUCGU- -5' |
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| 28028 | 3' | -54.7 | NC_005887.1 | + | 40935 | 0.68 | 0.551014 |
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Target: 5'- gCU-CGCGUUCGucuugCCGcUGCCGAGCGg -3' miRNA: 3'- -GAcGCGUAGGCua---GGCaACGGUUCGU- -5' |
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| 28028 | 3' | -54.7 | NC_005887.1 | + | 36282 | 0.68 | 0.555461 |
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Target: 5'- -cGCGCAUcuucguuggugagugCCGcgCCGcUGCCAccAGCAc -3' miRNA: 3'- gaCGCGUA---------------GGCuaGGCaACGGU--UCGU- -5' |
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| 28028 | 3' | -54.7 | NC_005887.1 | + | 35736 | 0.67 | 0.595887 |
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Target: 5'- -gGCGuUGUCgGAUCCGgUGCCGccAGCGu -3' miRNA: 3'- gaCGC-GUAGgCUAGGCaACGGU--UCGU- -5' |
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| 28028 | 3' | -54.7 | NC_005887.1 | + | 37701 | 0.66 | 0.623099 |
|
Target: 5'- uCUGCGCGUCgcugagauccuugauCGcgCCGgcgGCCAccGGCGc -3' miRNA: 3'- -GACGCGUAG---------------GCuaGGCaa-CGGU--UCGU- -5' |
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| 28028 | 3' | -54.7 | NC_005887.1 | + | 16858 | 0.66 | 0.652629 |
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Target: 5'- -cGCGCcaucGUCCGAgccgCCGcugcguugUGCCcAAGCAa -3' miRNA: 3'- gaCGCG----UAGGCUa---GGCa-------ACGG-UUCGU- -5' |
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| 28028 | 3' | -54.7 | NC_005887.1 | + | 25320 | 1.08 | 0.000817 |
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Target: 5'- gCUGCGCAUCCGAUCCGUUGCCAAGCAc -3' miRNA: 3'- -GACGCGUAGGCUAGGCAACGGUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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