Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28032 | 3' | -53 | NC_005887.1 | + | 36152 | 0.69 | 0.588852 |
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Target: 5'- -uCUCGCCgguuUCCucgUCGCGGcgcGUCAccgUGCCg -3' miRNA: 3'- uuGAGCGGa---AGG---AGCGCU---UAGU---ACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 36425 | 0.67 | 0.691855 |
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Target: 5'- cAC-CGCCUUCCgCGCGGccggcuUCuUGCCc -3' miRNA: 3'- uUGaGCGGAAGGaGCGCUu-----AGuACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 37793 | 0.67 | 0.703147 |
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Target: 5'- uGCUgCGCCUgcaguUCCUCGuCGGugcUCAUcGCCu -3' miRNA: 3'- uUGA-GCGGA-----AGGAGC-GCUu--AGUA-CGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 38046 | 0.67 | 0.691855 |
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Target: 5'- cAGCUgGCCggCCUCGgCGAGg-AUGUCg -3' miRNA: 3'- -UUGAgCGGaaGGAGC-GCUUagUACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 39726 | 0.7 | 0.488836 |
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Target: 5'- uGACUCGCCUcggCCacgUGCGAGUaCAUGUUu -3' miRNA: 3'- -UUGAGCGGAa--GGa--GCGCUUA-GUACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 40922 | 0.68 | 0.60028 |
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Target: 5'- ---aCGCCUUCCagcagcUCGCGuucGUCuUGCCg -3' miRNA: 3'- uugaGCGGAAGG------AGCGCu--UAGuACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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