Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28032 | 3' | -53 | NC_005887.1 | + | 18555 | 0.67 | 0.669094 |
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Target: 5'- cGGCggCGCCggCUUCGCGGGcgaCAUGCUc -3' miRNA: 3'- -UUGa-GCGGaaGGAGCGCUUa--GUACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 6852 | 0.67 | 0.691855 |
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Target: 5'- cGACgUGUCggaCCUCGCGAcgUcgGCCa -3' miRNA: 3'- -UUGaGCGGaa-GGAGCGCUuaGuaCGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 36425 | 0.67 | 0.691855 |
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Target: 5'- cAC-CGCCUUCCgCGCGGccggcuUCuUGCCc -3' miRNA: 3'- uUGaGCGGAAGGaGCGCUu-----AGuACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 4806 | 0.66 | 0.73652 |
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Target: 5'- uGCUCGCggaUCUCGCGcAGUUcUGCCg -3' miRNA: 3'- uUGAGCGgaaGGAGCGC-UUAGuACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 3966 | 0.66 | 0.758219 |
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Target: 5'- cGACcCGCCgUUgCUCGCGAAcgaggaggCGUGCUu -3' miRNA: 3'- -UUGaGCGG-AAgGAGCGCUUa-------GUACGG- -5' |
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| 28032 | 3' | -53 | NC_005887.1 | + | 30264 | 0.81 | 0.107028 |
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Target: 5'- cGCUUGCCgUCCggCGCGAcgAUCGUGCCg -3' miRNA: 3'- uUGAGCGGaAGGa-GCGCU--UAGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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