Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28041 | 3' | -46.5 | NC_005887.1 | + | 20867 | 0.7 | 0.919539 |
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Target: 5'- gCGGCGAu-GCgaGAUagGUCGAGAAACc -3' miRNA: 3'- -GCUGCUuuCGggCUAa-UAGCUCUUUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 30485 | 0.7 | 0.912839 |
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Target: 5'- aCGACGAAAGaccgCCGAgcGUCGA-AAACg -3' miRNA: 3'- -GCUGCUUUCg---GGCUaaUAGCUcUUUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 40471 | 0.7 | 0.912839 |
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Target: 5'- cCGGCGucgacGAGCUCGAUUA-CGAGGcguGGCg -3' miRNA: 3'- -GCUGCu----UUCGGGCUAAUaGCUCU---UUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 27669 | 0.71 | 0.874638 |
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Target: 5'- gCGACGA--GCCCGAUcGUgCGGGGcAGCg -3' miRNA: 3'- -GCUGCUuuCGGGCUAaUA-GCUCU-UUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 39178 | 0.72 | 0.829197 |
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Target: 5'- gCGGCagcuGCCCGAacUUGUCGAGgcGCg -3' miRNA: 3'- -GCUGcuuuCGGGCU--AAUAGCUCuuUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 30646 | 0.73 | 0.788454 |
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Target: 5'- gCGGCGAAGcgaucuugcgacGCCUGAUcGUCGAGcGACu -3' miRNA: 3'- -GCUGCUUU------------CGGGCUAaUAGCUCuUUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 11016 | 0.73 | 0.766894 |
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Target: 5'- uCGACGAcGGCCCGGUcggcaCGuGGGACg -3' miRNA: 3'- -GCUGCUuUCGGGCUAaua--GCuCUUUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 33755 | 0.74 | 0.744691 |
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Target: 5'- aCGGCGAGAGCUCGccagcGUCGAacGAAACc -3' miRNA: 3'- -GCUGCUUUCGGGCuaa--UAGCU--CUUUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 15705 | 0.76 | 0.627995 |
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Target: 5'- uCGACGAcAGCCCGA---UCGAGGccaugAACg -3' miRNA: 3'- -GCUGCUuUCGGGCUaauAGCUCU-----UUG- -5' |
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| 28041 | 3' | -46.5 | NC_005887.1 | + | 19854 | 1.11 | 0.005302 |
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Target: 5'- cCGACGAAAGCCCGAUUAUCGAGAAACc -3' miRNA: 3'- -GCUGCUUUCGGGCUAAUAGCUCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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