Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28058 | 5' | -55.7 | NC_005887.1 | + | 1440 | 0.71 | 0.314821 |
Target: 5'- --uGCGCGCGCG-GUagcccUUCGGCGGCu -3' miRNA: 3'- uauCGCGCGCGCaCAgc---AAGCUGCUG- -5' |
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28058 | 5' | -55.7 | NC_005887.1 | + | 28293 | 0.73 | 0.256431 |
Target: 5'- -gAGCGCGCGCaGcGUCGU--GGCGACu -3' miRNA: 3'- uaUCGCGCGCG-CaCAGCAagCUGCUG- -5' |
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28058 | 5' | -55.7 | NC_005887.1 | + | 1072 | 0.74 | 0.207214 |
Target: 5'- -cAGCGCGCcCGUG-CGcaggUCGACGGCg -3' miRNA: 3'- uaUCGCGCGcGCACaGCa---AGCUGCUG- -5' |
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28058 | 5' | -55.7 | NC_005887.1 | + | 33642 | 0.75 | 0.196256 |
Target: 5'- cGUAGCGCGCGC-UG-CGcUCGGCGAg -3' miRNA: 3'- -UAUCGCGCGCGcACaGCaAGCUGCUg -5' |
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28058 | 5' | -55.7 | NC_005887.1 | + | 39913 | 0.78 | 0.118604 |
Target: 5'- cGUAGCGCGCGagGUGUCaUUCGuCGGCg -3' miRNA: 3'- -UAUCGCGCGCg-CACAGcAAGCuGCUG- -5' |
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28058 | 5' | -55.7 | NC_005887.1 | + | 13629 | 1.07 | 0.000827 |
Target: 5'- gAUAGCGCGCGCGUGUCGUUCGACGACu -3' miRNA: 3'- -UAUCGCGCGCGCACAGCAAGCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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