Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28061 | 5' | -47.9 | NC_005887.1 | + | 27860 | 0.66 | 0.966145 |
Target: 5'- aAGCACCgcggcgGGCACGAucuGCGcGAGGcccgacACGAc -3' miRNA: 3'- gUUGUGGa-----UUGUGCU---UGC-CUCU------UGCU- -5' |
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28061 | 5' | -47.9 | NC_005887.1 | + | 29279 | 0.71 | 0.78242 |
Target: 5'- -uACACCUAACACGGACGuacAGcAACGc -3' miRNA: 3'- guUGUGGAUUGUGCUUGCc--UC-UUGCu -5' |
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28061 | 5' | -47.9 | NC_005887.1 | + | 34347 | 0.67 | 0.949213 |
Target: 5'- uGGCGCCggcCACGGcUGGAGA-CGAg -3' miRNA: 3'- gUUGUGGauuGUGCUuGCCUCUuGCU- -5' |
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28061 | 5' | -47.9 | NC_005887.1 | + | 35145 | 0.69 | 0.877326 |
Target: 5'- aCAGCGCCUGcgcACGCGAGaauucGAGAAgCGAg -3' miRNA: 3'- -GUUGUGGAU---UGUGCUUgc---CUCUU-GCU- -5' |
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28061 | 5' | -47.9 | NC_005887.1 | + | 39331 | 0.72 | 0.771749 |
Target: 5'- aCAGCauuACCUGACAUGAAaCGGGGGcaACGu -3' miRNA: 3'- -GUUG---UGGAUUGUGCUU-GCCUCU--UGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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