Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28079 | 3' | -61.6 | NC_005887.1 | + | 4231 | 0.66 | 0.327795 |
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Target: 5'- -gCG-UGCUGCUCGCGccgacgcUCGGCCGCAc -3' miRNA: 3'- ggGCaACGGCGGGCGUc------AGUCGGCGU- -5' |
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| 28079 | 3' | -61.6 | NC_005887.1 | + | 12981 | 0.66 | 0.335666 |
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Target: 5'- gCCGagggcaUGCCGCUCGCGG-CuGCCGgGu -3' miRNA: 3'- gGGCa-----ACGGCGGGCGUCaGuCGGCgU- -5' |
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| 28079 | 3' | -61.6 | NC_005887.1 | + | 30269 | 0.66 | 0.346915 |
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Target: 5'- gCCGUccggcgcgacgaucgUGCCGCgcacgacguUCGCAGUCAGgCCGa- -3' miRNA: 3'- gGGCA---------------ACGGCG---------GGCGUCAGUC-GGCgu -5' |
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| 28079 | 3' | -61.6 | NC_005887.1 | + | 41618 | 0.66 | 0.351817 |
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Target: 5'- gCCGgcGCgGCCCGUgaGGaUCuGCUGCGu -3' miRNA: 3'- gGGCaaCGgCGGGCG--UC-AGuCGGCGU- -5' |
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| 28079 | 3' | -61.6 | NC_005887.1 | + | 32835 | 0.66 | 0.351817 |
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Target: 5'- -aCGUgaugGCCGCCgUGCAgGUCAaGCgGCAu -3' miRNA: 3'- ggGCAa---CGGCGG-GCGU-CAGU-CGgCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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