Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28079 | 3' | -61.6 | NC_005887.1 | + | 28647 | 0.71 | 0.152365 |
Target: 5'- gCCGUUgaucugcugGCCGCCCGUGGUCGagaucccacccGUCGCGc -3' miRNA: 3'- gGGCAA---------CGGCGGGCGUCAGU-----------CGGCGU- -5' |
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28079 | 3' | -61.6 | NC_005887.1 | + | 5832 | 0.72 | 0.12953 |
Target: 5'- aCCUGccGCCGCCCgaugcuGCGGUCGaCCGCAc -3' miRNA: 3'- -GGGCaaCGGCGGG------CGUCAGUcGGCGU- -5' |
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28079 | 3' | -61.6 | NC_005887.1 | + | 7285 | 0.74 | 0.098388 |
Target: 5'- cCCCG--GCCGCCCGCuGUgGGUCGUc -3' miRNA: 3'- -GGGCaaCGGCGGGCGuCAgUCGGCGu -5' |
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28079 | 3' | -61.6 | NC_005887.1 | + | 19925 | 0.78 | 0.044671 |
Target: 5'- cCCUGacgcUGCCGCCCGguGUCAGCCu-- -3' miRNA: 3'- -GGGCa---ACGGCGGGCguCAGUCGGcgu -5' |
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28079 | 3' | -61.6 | NC_005887.1 | + | 8050 | 1.09 | 0.000162 |
Target: 5'- aCCCGUUGCCGCCCGCAGUCAGCCGCAc -3' miRNA: 3'- -GGGCAACGGCGGGCGUCAGUCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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