Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28079 | 5' | -55.1 | NC_005887.1 | + | 11630 | 0.66 | 0.60028 |
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Target: 5'- gCGUGCGCAAuGCCGagcuCGAcGCGUUGcAGAc -3' miRNA: 3'- -GUACGCGUU-CGGC----GCU-CGCGAC-UUUa -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 22977 | 0.66 | 0.611738 |
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Target: 5'- -cUGCGCGAGCauggCGCGuuGCcGCUGAu-- -3' miRNA: 3'- guACGCGUUCG----GCGCu-CG-CGACUuua -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 24615 | 0.66 | 0.611738 |
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Target: 5'- --cGCGC-GGCCGUgccGAGCGUcuUGAAGa -3' miRNA: 3'- guaCGCGuUCGGCG---CUCGCG--ACUUUa -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 9753 | 0.66 | 0.611738 |
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Target: 5'- uCGUGUGCGGGCUGCacguGCaCUGGAAc -3' miRNA: 3'- -GUACGCGUUCGGCGcu--CGcGACUUUa -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 18976 | 0.66 | 0.611738 |
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Target: 5'- --aGCGCGAGCagcaaaagcaucUGCGAGCGCcGcgGUg -3' miRNA: 3'- guaCGCGUUCG------------GCGCUCGCGaCuuUA- -5' |
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| 28079 | 5' | -55.1 | NC_005887.1 | + | 27474 | 0.66 | 0.611738 |
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Target: 5'- --gGCGCAuucuGCCGCGcggugcGCGCUGuuGc -3' miRNA: 3'- guaCGCGUu---CGGCGCu-----CGCGACuuUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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