Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28079 | 5' | -55.1 | NC_005887.1 | + | 24736 | 0.73 | 0.244181 |
Target: 5'- uCGUGCGCAGcguuuccaucuuGCgCGCGAGCGCgGGAu- -3' miRNA: 3'- -GUACGCGUU------------CG-GCGCUCGCGaCUUua -5' |
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28079 | 5' | -55.1 | NC_005887.1 | + | 18410 | 0.73 | 0.237649 |
Target: 5'- gCGUGCGCAgacGGCCGCGAG-GCcGAc-- -3' miRNA: 3'- -GUACGCGU---UCGGCGCUCgCGaCUuua -5' |
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28079 | 5' | -55.1 | NC_005887.1 | + | 3610 | 0.73 | 0.225023 |
Target: 5'- -uUGCGcCAGGCCGCGcAGCGCuucggccgUGAAAa -3' miRNA: 3'- guACGC-GUUCGGCGC-UCGCG--------ACUUUa -5' |
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28079 | 5' | -55.1 | NC_005887.1 | + | 3205 | 0.74 | 0.190527 |
Target: 5'- --aGCGagaaGGGCCGCGAGCGCUcgcaGAAGa -3' miRNA: 3'- guaCGCg---UUCGGCGCUCGCGA----CUUUa -5' |
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28079 | 5' | -55.1 | NC_005887.1 | + | 16276 | 0.78 | 0.097944 |
Target: 5'- --cGCGCGGGCCGCuGAcGCGCUGAc-- -3' miRNA: 3'- guaCGCGUUCGGCG-CU-CGCGACUuua -5' |
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28079 | 5' | -55.1 | NC_005887.1 | + | 8085 | 1.05 | 0.001114 |
Target: 5'- gCAUGCGCAAGCCGCGAGCGCUGAAAUc -3' miRNA: 3'- -GUACGCGUUCGGCGCUCGCGACUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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