Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2811 | 3' | -62.4 | NC_001491.2 | + | 114970 | 0.66 | 0.643577 |
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Target: 5'- gGCC-GCCCugACuCCGCGggucgccugcGCCGUGc- -3' miRNA: 3'- -CGGuCGGGugUG-GGCGCa---------CGGCACcc -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 117793 | 0.66 | 0.653302 |
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Target: 5'- uCCAGCUUcgGCGCCCGCucccaGCCGcccGGGa -3' miRNA: 3'- cGGUCGGG--UGUGGGCGca---CGGCa--CCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 118510 | 0.66 | 0.663011 |
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Target: 5'- cCCAGCCCgccGCGCCCGaCcUGCUGUacGGc -3' miRNA: 3'- cGGUCGGG---UGUGGGC-GcACGGCA--CCc -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 42559 | 0.66 | 0.663011 |
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Target: 5'- aGCCAGC--ACugCCGCGUauaucucgGCCGcagccUGGGc -3' miRNA: 3'- -CGGUCGggUGugGGCGCA--------CGGC-----ACCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 23377 | 0.66 | 0.663011 |
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Target: 5'- -aCGGCCCACGCUgucugcauggUGCuUGCCGccuuUGGGu -3' miRNA: 3'- cgGUCGGGUGUGG----------GCGcACGGC----ACCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 56672 | 0.66 | 0.67173 |
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Target: 5'- aCCAGUCCAgACagucuagCCGCGUacgcGCCGaGGGc -3' miRNA: 3'- cGGUCGGGUgUG-------GGCGCA----CGGCaCCC- -5' |
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| 2811 | 3' | -62.4 | NC_001491.2 | + | 106157 | 0.66 | 0.672698 |
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Target: 5'- gGUCGGCCagaCACGCCagugaaCGCG-GCCaUGGGa -3' miRNA: 3'- -CGGUCGG---GUGUGG------GCGCaCGGcACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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