Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28129 | 5' | -54.5 | NC_005887.1 | + | 37131 | 0.68 | 0.51785 |
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Target: 5'- cGCCCGGAAUcuUCugGuugaugccgaagacGAUCAGCcGCUu -3' miRNA: 3'- uCGGGCCUUA--AGugC--------------UUGGUCGaCGA- -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 31979 | 0.68 | 0.516761 |
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Target: 5'- cGGCCCGGAucuugcagcagCAUGccuGACCGGCUGg- -3' miRNA: 3'- -UCGGGCCUuaa--------GUGC---UUGGUCGACga -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 27311 | 0.69 | 0.447213 |
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Target: 5'- cGGgCCGGcg--CGCGAugCAGUUGCg -3' miRNA: 3'- -UCgGGCCuuaaGUGCUugGUCGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 15981 | 0.7 | 0.427175 |
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Target: 5'- cGGCCCgcgcGGAAcgCAgGucgGCCAGCUGCg -3' miRNA: 3'- -UCGGG----CCUUaaGUgCu--UGGUCGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 3617 | 0.7 | 0.407679 |
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Target: 5'- cAGgCCGGAauuGUUCucgGCGAACCAgaggcGCUGCa -3' miRNA: 3'- -UCgGGCCU---UAAG---UGCUUGGU-----CGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 36606 | 0.7 | 0.397196 |
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Target: 5'- cGCCugcCGGAAUUCGCGAaauaccugccgccGCCcgauGCUGCg -3' miRNA: 3'- uCGG---GCCUUAAGUGCU-------------UGGu---CGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 12890 | 0.72 | 0.303111 |
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Target: 5'- uGGUUCGuGAAUUC-CGGGCuCAGCUGCUu -3' miRNA: 3'- -UCGGGC-CUUAAGuGCUUG-GUCGACGA- -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 32861 | 0.73 | 0.285589 |
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Target: 5'- uGCCCGuGAagcggcgcgcgccgAUccugcgCACGAGCCGGCUGCa -3' miRNA: 3'- uCGGGC-CU--------------UAa-----GUGCUUGGUCGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 12829 | 1.08 | 0.000808 |
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Target: 5'- gAGCCCGGAAUUCACGAACCAGCUGCUg -3' miRNA: 3'- -UCGGGCCUUAAGUGCUUGGUCGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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