Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28129 | 5' | -54.5 | NC_005887.1 | + | 31734 | 0.68 | 0.557606 |
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Target: 5'- cGGCCCGGcuaacggcuucuggCGCGAugcugACUGGCUGCUc -3' miRNA: 3'- -UCGGGCCuuaa----------GUGCU-----UGGUCGACGA- -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 31979 | 0.68 | 0.516761 |
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Target: 5'- cGGCCCGGAucuugcagcagCAUGccuGACCGGCUGg- -3' miRNA: 3'- -UCGGGCCUuaa--------GUGC---UUGGUCGACga -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 32861 | 0.73 | 0.285589 |
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Target: 5'- uGCCCGuGAagcggcgcgcgccgAUccugcgCACGAGCCGGCUGCa -3' miRNA: 3'- uCGGGC-CU--------------UAa-----GUGCUUGGUCGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 35010 | 0.66 | 0.656233 |
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Target: 5'- cGCCCGccGAGUUCGa-AGCCGGCgacgGCa -3' miRNA: 3'- uCGGGC--CUUAAGUgcUUGGUCGa---CGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 35364 | 0.68 | 0.543134 |
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Target: 5'- cGCCCGGcaccaCGCGGGCCuGCaGCa -3' miRNA: 3'- uCGGGCCuuaa-GUGCUUGGuCGaCGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 36551 | 0.66 | 0.667589 |
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Target: 5'- cGGuUCCGGugaUCGCGAACCucGUUGCa -3' miRNA: 3'- -UC-GGGCCuuaAGUGCUUGGu-CGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 36606 | 0.7 | 0.397196 |
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Target: 5'- cGCCugcCGGAAUUCGCGAaauaccugccgccGCCcgauGCUGCg -3' miRNA: 3'- uCGG---GCCUUAAGUGCU-------------UGGu---CGACGa -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 37131 | 0.68 | 0.51785 |
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Target: 5'- cGCCCGGAAUcuUCugGuugaugccgaagacGAUCAGCcGCUu -3' miRNA: 3'- uCGGGCCUUA--AGugC--------------UUGGUCGaCGA- -5' |
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| 28129 | 5' | -54.5 | NC_005887.1 | + | 38258 | 0.66 | 0.656233 |
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Target: 5'- uGGCUCGGGuau--UGAGCCGGCcaUGCUg -3' miRNA: 3'- -UCGGGCCUuaaguGCUUGGUCG--ACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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