Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28151 | 3' | -58.2 | NC_005887.1 | + | 3169 | 0.66 | 0.450105 |
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Target: 5'- aCGACuGCCGCuuuuccuucaUGCGGCCgUggUCGGc -3' miRNA: 3'- -GUUG-CGGCGug--------ACGCCGG-GuuAGCCu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 11552 | 0.66 | 0.450105 |
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Target: 5'- uCGGCGCgcagCGCGCgGCGGUCgCGgaAUCGGGc -3' miRNA: 3'- -GUUGCG----GCGUGaCGCCGG-GU--UAGCCU- -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 1385 | 0.66 | 0.460029 |
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Target: 5'- -cGCGUCGCGCUcGUucaucaugGGCCCGcUCGGc -3' miRNA: 3'- guUGCGGCGUGA-CG--------CCGGGUuAGCCu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 372 | 0.66 | 0.470068 |
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Target: 5'- -uACGCgCGCACcGCGGCCUcgcuGAUCa-- -3' miRNA: 3'- guUGCG-GCGUGaCGCCGGG----UUAGccu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 24888 | 0.66 | 0.474115 |
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Target: 5'- cCGAUGCCGCGCgcuCGGUCUucuucgcagcagccgGAUCGGc -3' miRNA: 3'- -GUUGCGGCGUGac-GCCGGG---------------UUAGCCu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 27406 | 0.66 | 0.480217 |
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Target: 5'- -cGCGCCGCuGCgcGUGGCgCAGUCGa- -3' miRNA: 3'- guUGCGGCG-UGa-CGCCGgGUUAGCcu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 11428 | 0.66 | 0.480217 |
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Target: 5'- uGACGCCcGCGCUGaucaGGCUCAGUg--- -3' miRNA: 3'- gUUGCGG-CGUGACg---CCGGGUUAgccu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 37497 | 0.66 | 0.487385 |
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Target: 5'- aAGCGCCGCGCUccacuccgcaccugGCGuGCaggCAGUCGcGAc -3' miRNA: 3'- gUUGCGGCGUGA--------------CGC-CGg--GUUAGC-CU- -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 40743 | 0.65 | 0.497711 |
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Target: 5'- -uGCGCgCGCGCcaggugcaggauggUGCGGCCgguGAUCGGc -3' miRNA: 3'- guUGCG-GCGUG--------------ACGCCGGg--UUAGCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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