Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28151 | 3' | -58.2 | NC_005887.1 | + | 12660 | 0.71 | 0.233598 |
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Target: 5'- -cACGCCGaGCgucGCGGCCgGGUCGGc -3' miRNA: 3'- guUGCGGCgUGa--CGCCGGgUUAGCCu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 25020 | 0.71 | 0.233598 |
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Target: 5'- ---gGCCGCGCgcaagGCGGCCCGcgCGc- -3' miRNA: 3'- guugCGGCGUGa----CGCCGGGUuaGCcu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 14223 | 0.71 | 0.232987 |
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Target: 5'- gCGGCGCCGCGCUguucggcGCGGCauCCGAUCu-- -3' miRNA: 3'- -GUUGCGGCGUGA-------CGCCG--GGUUAGccu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 1493 | 0.72 | 0.188835 |
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Target: 5'- gCGGCGCuCGCGCggcGCGGCCguG-CGGAa -3' miRNA: 3'- -GUUGCG-GCGUGa--CGCCGGguUaGCCU- -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 30216 | 0.73 | 0.183306 |
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Target: 5'- -cACGCCGCGucGCGGCCUucgauguucgacaGGUCGGAc -3' miRNA: 3'- guUGCGGCGUgaCGCCGGG-------------UUAGCCU- -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 18037 | 0.73 | 0.16941 |
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Target: 5'- -cGCGCCGgACUGaCGGCCgaCGAUUGGGc -3' miRNA: 3'- guUGCGGCgUGAC-GCCGG--GUUAGCCU- -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 1065 | 0.73 | 0.164841 |
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Target: 5'- aCAGCGCgCGCACgGCGGCCgGcaauuugcgcguGUCGGc -3' miRNA: 3'- -GUUGCG-GCGUGaCGCCGGgU------------UAGCCu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 12058 | 0.74 | 0.139689 |
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Target: 5'- aGGCGCCGCGCUGCaGCa-GGUCGGc -3' miRNA: 3'- gUUGCGGCGUGACGcCGggUUAGCCu -5' |
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| 28151 | 3' | -58.2 | NC_005887.1 | + | 23308 | 1.09 | 0.000362 |
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Target: 5'- aCAACGCCGCACUGCGGCCCAAUCGGAg -3' miRNA: 3'- -GUUGCGGCGUGACGCCGGGUUAGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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