Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28152 | 5' | -52.8 | NC_005887.1 | + | 16466 | 0.69 | 0.558824 |
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Target: 5'- cAUGCGCUcGACCGcgcggcaggAUCCGGCGGGc- -3' miRNA: 3'- cUGCGCGA-CUGGCa--------UAGGCUGCUUuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 6928 | 0.7 | 0.536452 |
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Target: 5'- uGCGCGUUGGCCGacgucgcgagGUCCGACa---- -3' miRNA: 3'- cUGCGCGACUGGCa---------UAGGCUGcuuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 23654 | 0.71 | 0.482044 |
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Target: 5'- uACGCGCUGAUUGUcggagauaAUCCGGCGc--- -3' miRNA: 3'- cUGCGCGACUGGCA--------UAGGCUGCuuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 32363 | 0.71 | 0.440512 |
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Target: 5'- cGGCGCGCcGGCUGgc-CCGACGAu-- -3' miRNA: 3'- -CUGCGCGaCUGGCauaGGCUGCUuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 9890 | 0.72 | 0.391591 |
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Target: 5'- -cCGCGCUGACCGgcUgCGACGc--- -3' miRNA: 3'- cuGCGCGACUGGCauAgGCUGCuuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 21521 | 0.72 | 0.391591 |
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Target: 5'- cGACcCGCUGACCGUGcUCGACGcuGAg -3' miRNA: 3'- -CUGcGCGACUGGCAUaGGCUGCuuUU- -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 30269 | 0.73 | 0.382239 |
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Target: 5'- cGACGCgGCgUGGCUGg--CCGGCGAGAAg -3' miRNA: 3'- -CUGCG-CG-ACUGGCauaGGCUGCUUUU- -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 13578 | 0.73 | 0.363988 |
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Target: 5'- uGGCGCGCaUGcCCGUG-CCGGCGAc-- -3' miRNA: 3'- -CUGCGCG-ACuGGCAUaGGCUGCUuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 23599 | 1.07 | 0.001758 |
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Target: 5'- cGACGCGCUGACCGUAUCCGACGAAAAg -3' miRNA: 3'- -CUGCGCGACUGGCAUAGGCUGCUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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