Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28154 | 3' | -59.4 | NC_005887.1 | + | 12079 | 0.66 | 0.416745 |
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Target: 5'- gAGCgGCGuGCGuG-CGCCGGAUGucuGCa -3' miRNA: 3'- gUCGgUGC-CGCuCaGCGGCCUAU---CGc -5' |
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| 28154 | 3' | -59.4 | NC_005887.1 | + | 37106 | 0.66 | 0.416745 |
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Target: 5'- aCAGCCuCGGCGAucgcgcggccGUCGCcCGGAaucuucUGGUu -3' miRNA: 3'- -GUCGGuGCCGCU----------CAGCG-GCCU------AUCGc -5' |
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| 28154 | 3' | -59.4 | NC_005887.1 | + | 20798 | 0.66 | 0.426118 |
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Target: 5'- uGGUCACGGaaaaaUGCUGGAUGGCGu -3' miRNA: 3'- gUCGGUGCCgcucaGCGGCCUAUCGC- -5' |
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| 28154 | 3' | -59.4 | NC_005887.1 | + | 6989 | 0.66 | 0.426118 |
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Target: 5'- gCGGcCCAUGcCGAGUUGCgGGAUucgcucGGCGg -3' miRNA: 3'- -GUC-GGUGCcGCUCAGCGgCCUA------UCGC- -5' |
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| 28154 | 3' | -59.4 | NC_005887.1 | + | 37061 | 0.66 | 0.426118 |
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Target: 5'- aCGGCC-UGcGCGuGcUCGCCGGcgAGCu -3' miRNA: 3'- -GUCGGuGC-CGCuC-AGCGGCCuaUCGc -5' |
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| 28154 | 3' | -59.4 | NC_005887.1 | + | 42022 | 0.66 | 0.445232 |
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Target: 5'- -cGCCGCGcacgcaccacGCGAGcaCGCCGGGcAGCu -3' miRNA: 3'- guCGGUGC----------CGCUCa-GCGGCCUaUCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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