Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28154 | 3' | -59.4 | NC_005887.1 | + | 4622 | 0.69 | 0.264491 |
Target: 5'- -cGCCGCGGCGccgguGGcCGCCGGc--GCGa -3' miRNA: 3'- guCGGUGCCGC-----UCaGCGGCCuauCGC- -5' |
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28154 | 3' | -59.4 | NC_005887.1 | + | 14701 | 0.7 | 0.25787 |
Target: 5'- -cGUCGCGGCGGGcggGCCGGGcgcGGCGa -3' miRNA: 3'- guCGGUGCCGCUCag-CGGCCUa--UCGC- -5' |
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28154 | 3' | -59.4 | NC_005887.1 | + | 538 | 0.7 | 0.251386 |
Target: 5'- uCGGCCGCcGCGcGccgCGCCGGAUacAGCGc -3' miRNA: 3'- -GUCGGUGcCGCuCa--GCGGCCUA--UCGC- -5' |
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28154 | 3' | -59.4 | NC_005887.1 | + | 32422 | 0.72 | 0.164976 |
Target: 5'- uGGCCgGCGGCGAuGUgGCCGGc-GGCGa -3' miRNA: 3'- gUCGG-UGCCGCU-CAgCGGCCuaUCGC- -5' |
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28154 | 3' | -59.4 | NC_005887.1 | + | 8760 | 0.75 | 0.106198 |
Target: 5'- -cGCCGCGGCGGaUCGCCGGGcccuGCa -3' miRNA: 3'- guCGGUGCCGCUcAGCGGCCUau--CGc -5' |
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28154 | 3' | -59.4 | NC_005887.1 | + | 24261 | 1.07 | 0.000405 |
Target: 5'- gCAGCCACGGCGAGUCGCCGGAUAGCGc -3' miRNA: 3'- -GUCGGUGCCGCUCAGCGGCCUAUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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