Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28162 | 5' | -57.3 | NC_005887.1 | + | 28385 | 0.66 | 0.481965 |
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Target: 5'- cGGCuUCGcGGCUGUcgaucacGCGCaCGUcgCAGCc -3' miRNA: 3'- -UUGuAGC-CCGACA-------CGCG-GCGuaGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 32299 | 0.69 | 0.324595 |
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Target: 5'- -uCGUCGGGCcagccgGcGCGCCGCAgu-GCg -3' miRNA: 3'- uuGUAGCCCGa-----CaCGCGGCGUaguCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 34917 | 0.67 | 0.441246 |
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Target: 5'- gAACGUCGcGCUGUGCggaacuuGCCGuCGcCGGCu -3' miRNA: 3'- -UUGUAGCcCGACACG-------CGGC-GUaGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 35475 | 0.67 | 0.452253 |
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Target: 5'- cGACucgUGGGCgaaggccGUGCGCgggCGCAUCgAGCa -3' miRNA: 3'- -UUGua-GCCCGa------CACGCG---GCGUAG-UCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 35817 | 0.71 | 0.257766 |
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Target: 5'- ----aCGcGCUguguuccucGUGCGCCGCGUCGGCg -3' miRNA: 3'- uuguaGCcCGA---------CACGCGGCGUAGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 37374 | 0.67 | 0.456292 |
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Target: 5'- cAGCGUCGGcuuccuucuccuucaGCUG-GCGCUGCG-CGGUc -3' miRNA: 3'- -UUGUAGCC---------------CGACaCGCGGCGUaGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 38645 | 0.68 | 0.384939 |
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Target: 5'- cAACAUCGGGCcGUcguucuCGUCG-AUCAGCa -3' miRNA: 3'- -UUGUAGCCCGaCAc-----GCGGCgUAGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 40514 | 0.67 | 0.452253 |
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Target: 5'- cGGCAuUCGGGaucaUGUcgaGCGCCGauuugAUCAGCu -3' miRNA: 3'- -UUGU-AGCCCg---ACA---CGCGGCg----UAGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 41435 | 0.7 | 0.264599 |
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Target: 5'- cAGCGccucgUGGGCUaUGCGCUGC-UCGGCa -3' miRNA: 3'- -UUGUa----GCCCGAcACGCGGCGuAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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