Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28162 | 5' | -57.3 | NC_005887.1 | + | 316 | 0.68 | 0.394144 |
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Target: 5'- cGGCAUCaGGUcgagGCGCCGCAggucaaaCAGCa -3' miRNA: 3'- -UUGUAGcCCGaca-CGCGGCGUa------GUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 481 | 0.67 | 0.442241 |
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Target: 5'- cAACAgCGGGCgGcGCGgCGCGUCuucGCg -3' miRNA: 3'- -UUGUaGCCCGaCaCGCgGCGUAGu--CG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 40514 | 0.67 | 0.452253 |
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Target: 5'- cGGCAuUCGGGaucaUGUcgaGCGCCGauuugAUCAGCu -3' miRNA: 3'- -UUGU-AGCCCg---ACA---CGCGGCg----UAGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 35475 | 0.67 | 0.452253 |
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Target: 5'- cGACucgUGGGCgaaggccGUGCGCgggCGCAUCgAGCa -3' miRNA: 3'- -UUGua-GCCCGa------CACGCG---GCGUAG-UCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 14105 | 0.66 | 0.462388 |
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Target: 5'- cGGCAUCGucucaGGCUGcgccgGCGgCGCGcCGGCg -3' miRNA: 3'- -UUGUAGC-----CCGACa----CGCgGCGUaGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 13502 | 0.66 | 0.472641 |
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Target: 5'- gAACGUgcUGaGCgGUGCGCCGCucgucgCAGCa -3' miRNA: 3'- -UUGUA--GCcCGaCACGCGGCGua----GUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 4329 | 0.66 | 0.490328 |
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Target: 5'- -uCAUCGGGCcgagcaguucagacUgcGUGCgGCCgaGCGUCGGCg -3' miRNA: 3'- uuGUAGCCCG--------------A--CACG-CGG--CGUAGUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 3010 | 0.66 | 0.493482 |
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Target: 5'- gAugGUCGcGGCg--GUGCCGCGgcgcuugcgCAGCu -3' miRNA: 3'- -UugUAGC-CCGacaCGCGGCGUa--------GUCG- -5' |
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| 28162 | 5' | -57.3 | NC_005887.1 | + | 1310 | 0.7 | 0.285979 |
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Target: 5'- cGACGuuuUCGcGuGCUGaGCGCCGCG-CAGCg -3' miRNA: 3'- -UUGU---AGC-C-CGACaCGCGGCGUaGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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