Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28162 | 5' | -57.3 | NC_005887.1 | + | 35817 | 0.71 | 0.257766 |
Target: 5'- ----aCGcGCUguguuccucGUGCGCCGCGUCGGCg -3' miRNA: 3'- uuguaGCcCGA---------CACGCGGCGUAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 41435 | 0.7 | 0.264599 |
Target: 5'- cAGCGccucgUGGGCUaUGCGCUGC-UCGGCa -3' miRNA: 3'- -UUGUa----GCCCGAcACGCGGCGuAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 14200 | 0.7 | 0.281597 |
Target: 5'- --uGUCGGGCgcggccucggacagGgcgGCGCCGCGcuguUCGGCg -3' miRNA: 3'- uugUAGCCCGa-------------Ca--CGCGGCGU----AGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 32299 | 0.69 | 0.324595 |
Target: 5'- -uCGUCGGGCcagccgGcGCGCCGCAgu-GCg -3' miRNA: 3'- uuGUAGCCCGa-----CaCGCGGCGUaguCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 38645 | 0.68 | 0.384939 |
Target: 5'- cAACAUCGGGCcGUcguucuCGUCG-AUCAGCa -3' miRNA: 3'- -UUGUAGCCCGaCAc-----GCGGCgUAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 1232 | 0.67 | 0.412977 |
Target: 5'- ----gCGGGCgcUGCGCgGCGcUCAGCa -3' miRNA: 3'- uuguaGCCCGacACGCGgCGU-AGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 17790 | 0.67 | 0.422599 |
Target: 5'- --gGUCGuGGCUGU-CGUCGCAggGGCu -3' miRNA: 3'- uugUAGC-CCGACAcGCGGCGUagUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 17966 | 0.67 | 0.432355 |
Target: 5'- uGCG-CGGGCU---CGCCGgCAUCGGCc -3' miRNA: 3'- uUGUaGCCCGAcacGCGGC-GUAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 28185 | 1.1 | 0.000318 |
Target: 5'- cAACAUCGGGCUGUGCGCCGCAUCAGCg -3' miRNA: 3'- -UUGUAGCCCGACACGCGGCGUAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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