Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28167 | 5' | -62.7 | NC_005887.1 | + | 15628 | 0.73 | 0.098457 |
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Target: 5'- -cAGGCGGCCGggcucGCCGAucagGGCGCCGu -3' miRNA: 3'- caUCCGCCGGCagc--CGGCU----CUGCGGU- -5' |
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| 28167 | 5' | -62.7 | NC_005887.1 | + | 2703 | 0.73 | 0.095778 |
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Target: 5'- -cGGGCGGCaagCGGCCGAGcCGCgCGa -3' miRNA: 3'- caUCCGCCGgcaGCCGGCUCuGCG-GU- -5' |
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| 28167 | 5' | -62.7 | NC_005887.1 | + | 24542 | 0.73 | 0.095778 |
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Target: 5'- ---cGCGGCCGcaccuUCGGCCG-GGCGCCc -3' miRNA: 3'- caucCGCCGGC-----AGCCGGCuCUGCGGu -5' |
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| 28167 | 5' | -62.7 | NC_005887.1 | + | 22917 | 0.74 | 0.083389 |
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Target: 5'- --cGGCGGCCGUCGaugguaCCGAGAucUGCCGg -3' miRNA: 3'- cauCCGCCGGCAGCc-----GGCUCU--GCGGU- -5' |
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| 28167 | 5' | -62.7 | NC_005887.1 | + | 25074 | 0.74 | 0.083389 |
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Target: 5'- --cGGCGGCCGcCGGCgCGAgcuGACGCUc -3' miRNA: 3'- cauCCGCCGGCaGCCG-GCU---CUGCGGu -5' |
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| 28167 | 5' | -62.7 | NC_005887.1 | + | 30528 | 1.07 | 0.000184 |
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Target: 5'- cGUAGGCGGCCGUCGGCCGAGACGCCAc -3' miRNA: 3'- -CAUCCGCCGGCAGCCGGCUCUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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