miRNA: 3'- aaGUG-GGUgC-CGCCcCA--UCGCGCCGC- -5'
miRNA: 3'- aAGUGgGUGCCGCcccaUCGCGCCGC- -5'
miRNA: 3'- aaGUGGGUGCCGc---C-CCau-CGCGCCGC- -5'
miRNA: 3'- aaGUGGGUGcCGCC-CCAUc-GCGCCGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28171 | 5' | -63.4 | NC_005887.1 | + | 37425 | 0.66 | 0.246551 |
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Target: 5'- -gCACgCCAgGuGCGGaGUggAGCGCGGCGc -3' miRNA: 3'- aaGUG-GGUgC-CGCCcCA--UCGCGCCGC- -5' |
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| 28171 | 5' | -63.4 | NC_005887.1 | + | 2199 | 0.66 | 0.252819 |
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Target: 5'- cUCACaCGCGGCG----AGCGCGGCGc -3' miRNA: 3'- aAGUGgGUGCCGCcccaUCGCGCCGC- -5' |
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| 28171 | 5' | -63.4 | NC_005887.1 | + | 27363 | 0.66 | 0.252819 |
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Target: 5'- -aCGCCUGCGGCcugcGcGGccuGCGCGGCGu -3' miRNA: 3'- aaGUGGGUGCCGc---C-CCau-CGCGCCGC- -5' |
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| 28171 | 5' | -63.4 | NC_005887.1 | + | 12383 | 0.66 | 0.27241 |
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Target: 5'- -gCAUCCGC-GCGGcGGUGuuCGCGGCGc -3' miRNA: 3'- aaGUGGGUGcCGCC-CCAUc-GCGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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