Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28171 | 5' | -63.4 | NC_005887.1 | + | 26650 | 0.73 | 0.075721 |
Target: 5'- -aCGCCgGCGGCGGaaucauccGGccGCGCGGCGg -3' miRNA: 3'- aaGUGGgUGCCGCC--------CCauCGCGCCGC- -5' |
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28171 | 5' | -63.4 | NC_005887.1 | + | 33806 | 0.73 | 0.073633 |
Target: 5'- --gACCCACGGCcaccacgcGGGUgucaggucGGCGCGGCGa -3' miRNA: 3'- aagUGGGUGCCGc-------CCCA--------UCGCGCCGC- -5' |
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28171 | 5' | -63.4 | NC_005887.1 | + | 30982 | 0.75 | 0.050912 |
Target: 5'- cUUCGCCCACuguuCGGGGUgcgucucucgcguGGCGCGGCGc -3' miRNA: 3'- -AAGUGGGUGcc--GCCCCA-------------UCGCGCCGC- -5' |
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28171 | 5' | -63.4 | NC_005887.1 | + | 32723 | 1.05 | 0.000228 |
Target: 5'- gUUCACCCACGGCGGGGUAGCGCGGCGc -3' miRNA: 3'- -AAGUGGGUGCCGCCCCAUCGCGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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