Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28172 | 3' | -52.9 | NC_005887.1 | + | 23248 | 0.66 | 0.763068 |
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Target: 5'- gGCGGCGGU-CggCGcGCAaaaggcgcagcUCGUGGCg -3' miRNA: 3'- -CGCCGCCAcGaaGCaCGUa----------AGCAUCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 36531 | 0.66 | 0.769364 |
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Target: 5'- gGCGGCGGcagGUauUUCGcgaauuccggcagGCGUUCGUGcGCc -3' miRNA: 3'- -CGCCGCCa--CG--AAGCa------------CGUAAGCAU-CG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 21348 | 0.66 | 0.792974 |
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Target: 5'- cCGGCGG-GgUUCaacagcugaauagGUGCGagCGUGGCg -3' miRNA: 3'- cGCCGCCaCgAAG-------------CACGUaaGCAUCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 26103 | 0.66 | 0.79398 |
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Target: 5'- gGCGaCGGUGCc-CGUGCGcUCGccGGCc -3' miRNA: 3'- -CGCcGCCACGaaGCACGUaAGCa-UCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 840 | 0.66 | 0.797986 |
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Target: 5'- cGCGGCcgcgaagaaagccaaGGUGCc-CGUGgAcgCGUGGCc -3' miRNA: 3'- -CGCCG---------------CCACGaaGCACgUaaGCAUCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 30474 | 0.65 | 0.801963 |
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Target: 5'- -aGGCGGUGCgcgacgcggccCGUGCGUUgCGcaccGCa -3' miRNA: 3'- cgCCGCCACGaa---------GCACGUAA-GCau--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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