Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28172 | 3' | -52.9 | NC_005887.1 | + | 20965 | 0.72 | 0.405276 |
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Target: 5'- cCGGCGGUGCagCGaaaaucGCGUUCGUGaacGCa -3' miRNA: 3'- cGCCGCCACGaaGCa-----CGUAAGCAU---CG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 30604 | 0.73 | 0.377289 |
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Target: 5'- cCGGUGaucaUGCUguugCGUGCAUUCGUGcGCg -3' miRNA: 3'- cGCCGCc---ACGAa---GCACGUAAGCAU-CG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 25514 | 0.73 | 0.37185 |
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Target: 5'- cGCGGCGGgugGCggcauaaucgUCGccgccgagcggugcGCGUUCGUAGCu -3' miRNA: 3'- -CGCCGCCa--CGa---------AGCa-------------CGUAAGCAUCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 36866 | 0.74 | 0.350632 |
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Target: 5'- uGCGGcCGGUGCUgcgccaUCGgccUGCG-UCGUGGUg -3' miRNA: 3'- -CGCC-GCCACGA------AGC---ACGUaAGCAUCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 13893 | 0.78 | 0.191192 |
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Target: 5'- cCGGCucGUGCgcgUCG-GCAUUCGUGGCa -3' miRNA: 3'- cGCCGc-CACGa--AGCaCGUAAGCAUCG- -5' |
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| 28172 | 3' | -52.9 | NC_005887.1 | + | 33163 | 1.15 | 0.000469 |
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Target: 5'- cGCGGCGGUGCUUCGUGCAUUCGUAGCg -3' miRNA: 3'- -CGCCGCCACGAAGCACGUAAGCAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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