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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28210 | 3' | -42.6 | NC_005891.1 | + | 3411 | 0.66 | 0.999332 |
Target: 5'- ----cGcgGUAGAAGuUGACC-ACCUGa -3' miRNA: 3'- uuauaUuaCGUUUUC-ACUGGuUGGAC- -5' |
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28210 | 3' | -42.6 | NC_005891.1 | + | 23219 | 0.66 | 0.998632 |
Target: 5'- -------cGCGGGAcGUGGCCAGCCc- -3' miRNA: 3'- uuauauuaCGUUUU-CACUGGUUGGac -5' |
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28210 | 3' | -42.6 | NC_005891.1 | + | 10934 | 0.67 | 0.997389 |
Target: 5'- ----cAAUGCGAuuGGUGGCCAAaCCg- -3' miRNA: 3'- uuauaUUACGUUu-UCACUGGUU-GGac -5' |
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28210 | 3' | -42.6 | NC_005891.1 | + | 25529 | 0.68 | 0.995321 |
Target: 5'- --cGUAGUGCuccuGUGAgCCAACCa- -3' miRNA: 3'- uuaUAUUACGuuuuCACU-GGUUGGac -5' |
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28210 | 3' | -42.6 | NC_005891.1 | + | 883 | 1.07 | 0.017604 |
Target: 5'- cAAUAUAAUGCAAAAGUGACCAACCUGc -3' miRNA: 3'- -UUAUAUUACGUUUUCACUGGUUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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