Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28214 | 3' | -54.3 | NC_005891.1 | + | 12301 | 0.66 | 0.648138 |
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Target: 5'- -cCACUGGUGUGucggucacagcGGCG--ACGGCCAUa -3' miRNA: 3'- uaGUGACUACGC-----------CUGCauUGCCGGUA- -5' |
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| 28214 | 3' | -54.3 | NC_005891.1 | + | 4630 | 0.66 | 0.625048 |
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Target: 5'- -gCACUGGUugacccGCaGugGUggUGGCCAUa -3' miRNA: 3'- uaGUGACUA------CGcCugCAuuGCCGGUA- -5' |
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| 28214 | 3' | -54.3 | NC_005891.1 | + | 13431 | 0.68 | 0.500692 |
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Target: 5'- --gGCUGAauUGCGGugGU--UGGCCAc -3' miRNA: 3'- uagUGACU--ACGCCugCAuuGCCGGUa -5' |
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| 28214 | 3' | -54.3 | NC_005891.1 | + | 12796 | 0.69 | 0.437466 |
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Target: 5'- -gCACUGAUuggaagagaGCGGACGgAugGGUCGUg -3' miRNA: 3'- uaGUGACUA---------CGCCUGCaUugCCGGUA- -5' |
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| 28214 | 3' | -54.3 | NC_005891.1 | + | 19375 | 0.7 | 0.407616 |
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Target: 5'- -cCACUGGUaaCGGA-GUGACGGCCAc -3' miRNA: 3'- uaGUGACUAc-GCCUgCAUUGCCGGUa -5' |
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| 28214 | 3' | -54.3 | NC_005891.1 | + | 4524 | 1.03 | 0.001728 |
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Target: 5'- cAUCACUGAUGCGGACGUAACGGCCAUc -3' miRNA: 3'- -UAGUGACUACGCCUGCAUUGCCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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