miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28218 5' -42.4 NC_005891.1 + 25914 0.66 0.999778
Target:  5'- ------cGAGUCAGUGUccUCGGAcGGCu -3'
miRNA:   3'- guacuuuUUCAGUUACA--AGCCU-UCGu -5'
28218 5' -42.4 NC_005891.1 + 31567 0.67 0.999173
Target:  5'- aGUGAAGAAGaCAgaGUGUUUGGAcuuGCc -3'
miRNA:   3'- gUACUUUUUCaGU--UACAAGCCUu--CGu -5'
28218 5' -42.4 NC_005891.1 + 29511 0.67 0.999173
Target:  5'- -uUGGGuGAGUCGAUGUaC-GAAGCAg -3'
miRNA:   3'- guACUUuUUCAGUUACAaGcCUUCGU- -5'
28218 5' -42.4 NC_005891.1 + 28368 0.75 0.901319
Target:  5'- gAUGAAcuGGcugCuAUGUUCGGAGGCAa -3'
miRNA:   3'- gUACUUuuUCa--GuUACAAGCCUUCGU- -5'
28218 5' -42.4 NC_005891.1 + 13073 0.87 0.318365
Target:  5'- -uUGAugGAGUUAAUGUUCGGAAGUAg -3'
miRNA:   3'- guACUuuUUCAGUUACAAGCCUUCGU- -5'
28218 5' -42.4 NC_005891.1 + 13041 1.12 0.0108
Target:  5'- aCAUGAAAAAGUCAAUGUUCGGAAGCAa -3'
miRNA:   3'- -GUACUUUUUCAGUUACAAGCCUUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.