Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28218 | 5' | -42.4 | NC_005891.1 | + | 13041 | 1.12 | 0.0108 |
Target: 5'- aCAUGAAAAAGUCAAUGUUCGGAAGCAa -3' miRNA: 3'- -GUACUUUUUCAGUUACAAGCCUUCGU- -5' |
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28218 | 5' | -42.4 | NC_005891.1 | + | 13073 | 0.87 | 0.318365 |
Target: 5'- -uUGAugGAGUUAAUGUUCGGAAGUAg -3' miRNA: 3'- guACUuuUUCAGUUACAAGCCUUCGU- -5' |
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28218 | 5' | -42.4 | NC_005891.1 | + | 28368 | 0.75 | 0.901319 |
Target: 5'- gAUGAAcuGGcugCuAUGUUCGGAGGCAa -3' miRNA: 3'- gUACUUuuUCa--GuUACAAGCCUUCGU- -5' |
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28218 | 5' | -42.4 | NC_005891.1 | + | 31567 | 0.67 | 0.999173 |
Target: 5'- aGUGAAGAAGaCAgaGUGUUUGGAcuuGCc -3' miRNA: 3'- gUACUUUUUCaGU--UACAAGCCUu--CGu -5' |
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28218 | 5' | -42.4 | NC_005891.1 | + | 29511 | 0.67 | 0.999173 |
Target: 5'- -uUGGGuGAGUCGAUGUaC-GAAGCAg -3' miRNA: 3'- guACUUuUUCAGUUACAaGcCUUCGU- -5' |
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28218 | 5' | -42.4 | NC_005891.1 | + | 25914 | 0.66 | 0.999778 |
Target: 5'- ------cGAGUCAGUGUccUCGGAcGGCu -3' miRNA: 3'- guacuuuUUCAGUUACA--AGCCU-UCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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