Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28219 | 3' | -48.9 | NC_005891.1 | + | 9816 | 0.7 | 0.717044 |
Target: 5'- --cGCGGCACuggacgGGCUACAAGCAUCUa- -3' miRNA: 3'- uguCGUCGUG------UUGAUGUUUGUGGAcg -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 13751 | 1.12 | 0.001603 |
Target: 5'- gACAGCAGCACAACUACAAACACCUGCc -3' miRNA: 3'- -UGUCGUCGUGUUGAUGUUUGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 21374 | 0.66 | 0.917892 |
Target: 5'- gGCAGCAGgAUGGCUAC---CAUgUGCc -3' miRNA: 3'- -UGUCGUCgUGUUGAUGuuuGUGgACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 23597 | 0.66 | 0.911052 |
Target: 5'- cGCAGUAGCGaGACUGCGc-CGCCacuucgauuuggUGCa -3' miRNA: 3'- -UGUCGUCGUgUUGAUGUuuGUGG------------ACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 35872 | 0.66 | 0.908938 |
Target: 5'- uGCuGCAGCGCAACggcGCGGAUugaACCauucuuuuuggcugUGCg -3' miRNA: 3'- -UGuCGUCGUGUUGa--UGUUUG---UGG--------------ACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 21323 | 0.67 | 0.872098 |
Target: 5'- aAUGGCGGUGCGACUugGAcagggaaaaACAUCgGCu -3' miRNA: 3'- -UGUCGUCGUGUUGAugUU---------UGUGGaCG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 1454 | 0.68 | 0.835622 |
Target: 5'- cGCAGCuucGCGuaggUAACUAUAAGCACauaCUGCc -3' miRNA: 3'- -UGUCGu--CGU----GUUGAUGUUUGUG---GACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 3617 | 0.68 | 0.835622 |
Target: 5'- -aGGCGGUu--ACUACgAAAUGCCUGCc -3' miRNA: 3'- ugUCGUCGuguUGAUG-UUUGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 28373 | 0.68 | 0.825843 |
Target: 5'- -aGGUAGUcaaGGCUAUAGuCACCUGCu -3' miRNA: 3'- ugUCGUCGug-UUGAUGUUuGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 5525 | 0.68 | 0.824852 |
Target: 5'- aACAGaCGGCACAagcguuGCUucaaaaaACGGGCGcuCCUGCg -3' miRNA: 3'- -UGUC-GUCGUGU------UGA-------UGUUUGU--GGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 11127 | 0.69 | 0.784449 |
Target: 5'- uCGGCGGCGCAggcggcaauaACUccGCGAACACaggcgGCg -3' miRNA: 3'- uGUCGUCGUGU----------UGA--UGUUUGUGga---CG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 10624 | 0.69 | 0.784449 |
Target: 5'- uGCAGaaCGGCGgAACUACuuAUACCUcGCc -3' miRNA: 3'- -UGUC--GUCGUgUUGAUGuuUGUGGA-CG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 8782 | 0.72 | 0.610173 |
Target: 5'- gUAGuCGGCGCucuugcuACUACGAACGCC-GCg -3' miRNA: 3'- uGUC-GUCGUGu------UGAUGUUUGUGGaCG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 15024 | 0.71 | 0.634094 |
Target: 5'- cGCAGCAGgGuCGGCgaaguCAAACGgCUGCa -3' miRNA: 3'- -UGUCGUCgU-GUUGau---GUUUGUgGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 37271 | 0.71 | 0.658006 |
Target: 5'- -uGGUcGCAgAGC-ACAAACACCUGUa -3' miRNA: 3'- ugUCGuCGUgUUGaUGUUUGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 33474 | 0.7 | 0.693626 |
Target: 5'- -uGGCGGCAgGuagaUGCGAugacGCACCUGCc -3' miRNA: 3'- ugUCGUCGUgUug--AUGUU----UGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 29868 | 0.7 | 0.728612 |
Target: 5'- gACAGCugAGCGCAAC-GCAGAUACaaacgGCu -3' miRNA: 3'- -UGUCG--UCGUGUUGaUGUUUGUGga---CG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 11048 | 0.7 | 0.740067 |
Target: 5'- -aAGUAGUAC--CUACGuucgguGCACCUGCg -3' miRNA: 3'- ugUCGUCGUGuuGAUGUu-----UGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 16328 | 0.68 | 0.845148 |
Target: 5'- uCAGCGGCuccAUggUUugGGaagcgaacGCACCUGCa -3' miRNA: 3'- uGUCGUCG---UGuuGAugUU--------UGUGGACG- -5' |
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28219 | 3' | -48.9 | NC_005891.1 | + | 21715 | 0.67 | 0.863397 |
Target: 5'- cAUAGCuucCACAGauuCAGACGCCUGUc -3' miRNA: 3'- -UGUCGuc-GUGUUgauGUUUGUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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