miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28219 3' -48.9 NC_005891.1 + 15466 0.67 0.869518
Target:  5'- -gGGCAGCAUacgcagcuuugaugGACUcCAu-CACCUGCc -3'
miRNA:   3'- ugUCGUCGUG--------------UUGAuGUuuGUGGACG- -5'
28219 3' -48.9 NC_005891.1 + 36615 0.67 0.872098
Target:  5'- gAUAGCGGCAUuuuauGCUGCAGA-GCUUGa -3'
miRNA:   3'- -UGUCGUCGUGu----UGAUGUUUgUGGACg -5'
28219 3' -48.9 NC_005891.1 + 39196 0.67 0.888611
Target:  5'- cGCGGCcuGUcCAGCUACuuGCgGCCUGUc -3'
miRNA:   3'- -UGUCGu-CGuGUUGAUGuuUG-UGGACG- -5'
28219 3' -48.9 NC_005891.1 + 10427 0.67 0.888611
Target:  5'- uGCuGCGGCuacagACAAUgugGCcgucguAGCGCCUGCg -3'
miRNA:   3'- -UGuCGUCG-----UGUUGa--UGu-----UUGUGGACG- -5'
28219 3' -48.9 NC_005891.1 + 38667 0.66 0.911052
Target:  5'- -gAGCGGCAC-ACUcaACAAGUACCgccGCa -3'
miRNA:   3'- ugUCGUCGUGuUGA--UGUUUGUGGa--CG- -5'
28219 3' -48.9 NC_005891.1 + 8782 0.72 0.610173
Target:  5'- gUAGuCGGCGCucuugcuACUACGAACGCC-GCg -3'
miRNA:   3'- uGUC-GUCGUGu------UGAUGUUUGUGGaCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.