Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28219 | 3' | -48.9 | NC_005891.1 | + | 10427 | 0.67 | 0.888611 |
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Target: 5'- uGCuGCGGCuacagACAAUgugGCcgucguAGCGCCUGCg -3' miRNA: 3'- -UGuCGUCG-----UGUUGa--UGu-----UUGUGGACG- -5' |
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| 28219 | 3' | -48.9 | NC_005891.1 | + | 39196 | 0.67 | 0.888611 |
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Target: 5'- cGCGGCcuGUcCAGCUACuuGCgGCCUGUc -3' miRNA: 3'- -UGUCGu-CGuGUUGAUGuuUG-UGGACG- -5' |
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| 28219 | 3' | -48.9 | NC_005891.1 | + | 35872 | 0.66 | 0.908938 |
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Target: 5'- uGCuGCAGCGCAACggcGCGGAUugaACCauucuuuuuggcugUGCg -3' miRNA: 3'- -UGuCGUCGUGUUGa--UGUUUG---UGG--------------ACG- -5' |
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| 28219 | 3' | -48.9 | NC_005891.1 | + | 38667 | 0.66 | 0.911052 |
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Target: 5'- -gAGCGGCAC-ACUcaACAAGUACCgccGCa -3' miRNA: 3'- ugUCGUCGUGuUGA--UGUUUGUGGa--CG- -5' |
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| 28219 | 3' | -48.9 | NC_005891.1 | + | 23597 | 0.66 | 0.911052 |
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Target: 5'- cGCAGUAGCGaGACUGCGc-CGCCacuucgauuuggUGCa -3' miRNA: 3'- -UGUCGUCGUgUUGAUGUuuGUGG------------ACG- -5' |
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| 28219 | 3' | -48.9 | NC_005891.1 | + | 21374 | 0.66 | 0.917892 |
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Target: 5'- gGCAGCAGgAUGGCUAC---CAUgUGCc -3' miRNA: 3'- -UGUCGUCgUGUUGAUGuuuGUGgACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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