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miRNA: 3'- -CCUUUAUUAGGAAAACCUCACGGAAA- -5'
miRNA: 3'- -CCUUUAUUAGGAAAACCUCACGGAaa -5'
miRNA: 3'- cCUUua-UUAGGAaa---ACCUCACGGAaa -5'
miRNA: 3'- ccuuuaUUAGGAaa---ACCUCACGGAaa -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 28264 | 5' | -43.3 | NC_005902.1 | + | 61061 | 1.1 | 0.049233 |
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Target: 5'- uGGAAAUAAUCCUUUUGGAGUGCCUUUg -3' miRNA: 3'- -CCUUUAUUAGGAAAACCUCACGGAAA- -5' |
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| 28264 | 5' | -43.3 | NC_005902.1 | + | 61001 | 1.05 | 0.091371 |
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Target: 5'- uGGAAAUAAUCCUUUUGGAGUGCCUc- -3' miRNA: 3'- -CCUUUAUUAGGAAAACCUCACGGAaa -5' |
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| 28264 | 5' | -43.3 | NC_005902.1 | + | 124947 | 0.74 | 0.996709 |
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Target: 5'- aGAAuuaAAUCCUgguugUGGAGUGCCUc- -3' miRNA: 3'- cCUUua-UUAGGAaa---ACCUCACGGAaa -5' |
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| 28264 | 5' | -43.3 | NC_005902.1 | + | 125013 | 0.73 | 0.998717 |
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Target: 5'- -----aAAUCCUgguugUGGAGUGCCUc- -3' miRNA: 3'- ccuuuaUUAGGAaa---ACCUCACGGAaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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