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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28264 | 5' | -43.3 | NC_005902.1 | + | 125013 | 0.73 | 0.998717 |
Target: 5'- -----aAAUCCUgguugUGGAGUGCCUc- -3' miRNA: 3'- ccuuuaUUAGGAaa---ACCUCACGGAaa -5' |
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28264 | 5' | -43.3 | NC_005902.1 | + | 124947 | 0.74 | 0.996709 |
Target: 5'- aGAAuuaAAUCCUgguugUGGAGUGCCUc- -3' miRNA: 3'- cCUUua-UUAGGAaa---ACCUCACGGAaa -5' |
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28264 | 5' | -43.3 | NC_005902.1 | + | 61001 | 1.05 | 0.091371 |
Target: 5'- uGGAAAUAAUCCUUUUGGAGUGCCUc- -3' miRNA: 3'- -CCUUUAUUAGGAAAACCUCACGGAaa -5' |
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28264 | 5' | -43.3 | NC_005902.1 | + | 61061 | 1.1 | 0.049233 |
Target: 5'- uGGAAAUAAUCCUUUUGGAGUGCCUUUg -3' miRNA: 3'- -CCUUUAUUAGGAAAACCUCACGGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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